Valéry Céline, Paternostre Maïté, Robert Bruno, Gulik-Krzywicki Thaddée, Narayanan Theyencheri, Dedieu Jean-Claude, Keller Gérard, Torres Maria-Luisa, Cherif-Cheikh Roland, Calvo Pilar, Artzner Franck
Unité Mixte de Recherche 8612, Centre National de la Recherche Scientifique, Faculté de Pharmacie, 5 Rue J.B. Clément, 92296 Châtenay-Malabry Cedex, France.
Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10258-62. doi: 10.1073/pnas.1730609100. Epub 2003 Aug 20.
The controlled self-assembly of complex molecules into well defined hierarchical structures is a promising route for fabricating nanostructures. These nanoscale structures can be realized by naturally occurring proteins such as tobacco mosaic virus, capsid proteins, tubulin, actin, etc. Here, we report a simple alternative method based on self-assembling nanotubes formed by a synthetic therapeutic octapeptide, Lanreotide in water. We used a multidisciplinary approach involving optical and electron microscopies, vibrational spectroscopies, and small and wide angle x-ray scattering to elucidate the hierarchy of structures exhibited by this system. The results revealed the hexagonal packing of nanotubes, and high degree of monodispersity in the tube diameter (244 A) and wall thickness (approximately equal to 18 A). Moreover, the diameter is tunable by suitable modifications in the molecular structure. The self-assembly of the nanotubes occurs through the association of beta-sheets driven by amphiphilicity and a systematic aromatic/aliphatic side chain segregation. This original and simple system is a unique example for the study of complex self-assembling processes generated by de novo molecules or amyloid peptides.
将复杂分子可控地自组装成结构明确的分层结构是制造纳米结构的一条很有前景的途径。这些纳米级结构可以通过天然存在的蛋白质来实现，如烟草花叶病毒、衣壳蛋白、微管蛋白、肌动蛋白等。在此，我们报告一种基于合成治疗性八肽兰瑞肽在水中形成自组装纳米管的简单替代方法。我们采用了多学科方法，包括光学和电子显微镜、振动光谱以及小角和广角X射线散射，以阐明该系统所呈现的结构层次。结果揭示了纳米管的六边形堆积，以及在管直径（244 Å）和壁厚（约等于18 Å）方面的高度单分散性。此外，通过对分子结构进行适当修饰，直径是可调的。纳米管的自组装通过两亲性驱动的β-折叠片层的缔合以及系统的芳香族/脂肪族侧链分离而发生。这个原始且简单的系统是研究由从头合成分子或淀粉样肽产生的复杂自组装过程的一个独特例子。
