Jortani S A, Poklis A
Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0597.
J Anal Toxicol. 1992 Nov-Dec;16(6):368-71. doi: 10.1093/jat/16.6.368.
We evaluated the enzymatic Emit ETS Plus Ethyl Alcohol Assay (Emit) intended for the quantitative analysis of ethanol in human urine, serum, and plasma. The assay had been designed for use with the new Syva ETS Plus analyzer. The assay had a linear range up to 6.5 g/L and a low detection limit of 0.1 g/L. Assay within-run precision in serum with ethanol added to 0.25, 0.40, 1.00, 3.00, and 5.00 g/L yielded CVs (n = 32) of 6.1, 5.8, 3.7, 3.5, and 4.5%, respectively. Within-run precision of the assay using with ethanol added to 0.25, 0.40, 1.00, 3.00, and 5.00 g/L yielded CVs (n = 32) of 4.9, 5.0, 4.3, 3.3, and 5.0%, respectively. Between-run precision of aqueous controls yielded 0.41 +/- 0.02 g/L ethanol (target value, 0.40 g/L), CV = 4.5% (n = 31) and 3.05 +/- 0.06 g/L ethanol (target value, 3.00 g/L) CV = 2.1% (n = 31). Absolute recovery of the assay with 0.40 to 6.00 g/L ethanol added to serum and urine yielded mean recovery values of 105.5 and 101.5%, respectively. Results of the analysis of patient serum and urine specimens for ethanol by the Emit assay correlated well with those obtained by other methods. The Emit assay was found to be rapid, precise, and accurate for the determination of ethanol in clinical specimens. It was also found to be free of interferences from small molecular weight alcohols, aldehydes, ketones, and glycols. The Emit assay's linear range of up to 6.50 g/L ethanol was a major advantage over the ADx and aca assays; these assays were only linear to 3.00 g/L.
我们评估了用于定量分析人尿液、血清和血浆中乙醇的酶促埃米特ETS Plus乙醇测定法（Emit）。该测定法专为与新的Syva ETS Plus分析仪配合使用而设计。该测定法的线性范围高达6.5 g/L，检测下限低至0.1 g/L。在添加乙醇至0.25、0.40、1.00、3.00和5.00 g/L的血清中进行批内精密度测定，变异系数（n = 32）分别为6.1%、5.8%、3.7%、3.5%和4.5%。在添加乙醇至0.25、0.40、1.00、3.00和5.00 g/L的情况下使用该测定法进行批内精密度测定，变异系数（n = 32）分别为4.9%、5.0%、4.3%、3.3%和5.0%。水性对照的批间精密度得出乙醇含量为0.41±0.02 g/L（目标值为0.40 g/L），变异系数= 4.5%（n = 31），以及乙醇含量为3.05±0.06 g/L（目标值为3.00 g/L），变异系数= 2.1%（n = 31）。在血清和尿液中添加0.40至6.00 g/L乙醇时，该测定法的绝对回收率分别得出平均回收率为105.5%和101.5%。通过Emit测定法分析患者血清和尿液样本中乙醇的结果与通过其他方法获得的结果相关性良好。发现Emit测定法在测定临床样本中的乙醇时快速、精密且准确。还发现它不受小分子醇、醛、酮和二醇的干扰。Emit测定法高达6.50 g/L乙醇的线性范围是优于ADx和aca测定法的主要优势；这些测定法仅在3.00 g/L范围内呈线性。
