Jortani S A, Poklis A
Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond.
J Anal Toxicol. 1993 Sep;17(5):307-9. doi: 10.1093/jat/17.5.307.
The Abbott ADx REA assay (ADx) for the determination of ethanol in human serum and urine was evaluated. The within-run precision of a urine sample containing 3.00 g/L ethanol resulted in a coefficient of variance (CV) of 2.1% (n = 20). The within-run precision of a serum sample containing 1.47 g/L ethanol resulted in a CV of 2.3% (n = 20). The between-run precision of the TDx REA ethanol assay controls yielded 0.50 +/- 0.02 g/L ethanol (target value, 0.50 g/L) with a CV of 3.0% (n = 6); 1.03 +/- 0.03 g/L ethanol (target value, 1.00 g/L) with a CV of 2.7% (n = 6); and 2.56 +/- 0.07 g/L ethanol (target value, 2.50 g/L) with a CV of 2.8% (n = 6). Results of an intermethod comparison study of clinical serum and urine ethanol determinations by gas chromatography (x) and ADx (y) yielded by regression analysis y = 0.993x - 0.04 g/L (r = 0.985; n = 92) for serums and y = 0.974x - 0.02 g/L, (r = 0.991; n = 75) for urines. No significant interference was seen with low molecular weight alcohols or ketones.
对用于测定人血清和尿液中乙醇含量的雅培ADx REA检测法（ADx）进行了评估。一份含3.00 g/L乙醇的尿液样本的批内精密度，其变异系数（CV）为2.1%（n = 20）。一份含1.47 g/L乙醇的血清样本的批内精密度，其CV为2.3%（n = 20）。TDx REA乙醇检测法对照品的批间精密度，乙醇含量为0.50 +/- 0.02 g/L（目标值0.50 g/L），CV为3.0%（n = 6）；乙醇含量为1.03 +/- 0.03 g/L（目标值1.00 g/L），CV为2.7%（n = 6）；乙醇含量为2.56 +/- 0.07 g/L（目标值2.50 g/L），CV为2.8%（n = 6）。通过气相色谱法（x）和ADx（y）对临床血清和尿液乙醇测定进行的方法间比较研究结果，血清样本经回归分析得出y = 0.993x - 0.04 g/L（r = 0.985；n = 92），尿液样本得出y = 0.974x - 0.02 g/L（r = 0.991；n = 75）。未观察到低分子量醇类或酮类有明显干扰。
