通过铜绿假单胞菌PAO1的膜泡将庆大霉素递送至洋葱伯克霍尔德菌Ⅲa群菌株。
Gentamicin delivery to Burkholderia cepacia group IIIa strains via membrane vesicles from Pseudomonas aeruginosa PAO1.
作者信息
Allan Nick D, Beveridge Terry J
机构信息
Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
出版信息
Antimicrob Agents Chemother. 2003 Sep;47(9):2962-5. doi: 10.1128/AAC.47.9.2962-2965.2003.
Abstract
When Pseudomonas aeruginosa PAO1 is treated with gentamicin, it releases membrane vesicles containing gentamicin (g-MVs) and peptidoglycan hydrolase, which makes the MVs bactericidal. We evaluate the ability of g-MVs to deliver gentamicin past the intrinsic permeability barrier of group IIIa Burkholderia cepacia and show that strain CEP0248 with low resistance to gentamicin is killed but the highly resistant strain C5424 is not. Immunoelectron microscopy revealed that gentamicin was delivered into both strains, suggesting that there might be another mechanism of resistance in C5424.
摘要
当铜绿假单胞菌PAO1用庆大霉素处理时，它会释放出含有庆大霉素的膜泡（g-MVs）和肽聚糖水解酶，这使得这些膜泡具有杀菌作用。我们评估了g-MVs将庆大霉素递送至Ⅲa群洋葱伯克霍尔德菌固有通透性屏障之外的能力，并表明对庆大霉素低抗性的CEP0248菌株被杀死，但高抗性菌株C5424未被杀死。免疫电子显微镜显示庆大霉素被递送至两种菌株中，这表明C5424中可能存在另一种抗性机制。
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