Pereira Valéria Rêgo Alves, de Lorena Virginia Maria Barros, Nakazawa Mineo, da Silva Ana Paula Galvão, Montarroyos Ulisses, Correa-Oliveira Rodrigo, Gomes Yara de Miranda
Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil.
Rev Soc Bras Med Trop. 2003 Jul-Aug;36(4):435-40. doi: 10.1590/s0037-86822003000400001. Epub 2003 Aug 13.
Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypanosoma cruzi recombinant antigens CRA and FRA. Both antigens induced cutaneous immediate-type hypersensitivity response. The levels of IgG1, IgG2a, IgG2b and IgG3 were high in CRA immunized mice. IgG3 was the predominant isotype. Although no difference in antibody levels was observed in FRA-immunized mice when compared to control mice, both antigens were able to induce lymphoproliferation in immunized mice. Significant differences were observed between incorporation of [ H]- thymidine by spleen cell stimulated in vitro with CRA or FRA and the control group. These results suggest that CRA and FRA could be involved in mechanisms of resistance to Trypanosoma cruzi infection.
在44只接种克氏锥虫重组抗原CRA和FRA的C57BL/6小鼠中评估了体液免疫和细胞免疫反应。两种抗原均诱导了皮肤速发型超敏反应。接种CRA的小鼠中IgG1、IgG2a、IgG2b和IgG3水平较高。IgG3是主要的同种型。虽然与对照小鼠相比，接种FRA的小鼠在抗体水平上没有差异，但两种抗原均能诱导接种小鼠的淋巴细胞增殖。在用CRA或FRA体外刺激的脾细胞与对照组之间，观察到[H]-胸苷掺入存在显著差异。这些结果表明，CRA和FRA可能参与了对克氏锥虫感染的抗性机制。
