Pereira Valéria R A, Lorena Virginia Maria B, Verçosa Alinne Fernanda A, Silva Edmilson D, Ferreira Antonio G P, Montarroyos Ulisses R, Silva Ana P Galvão, Gomes Yara M
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães-Fiocruz, 50670-420 Recife, PE, Brasil.
Mem Inst Oswaldo Cruz. 2003 Sep;98(6):823-5. doi: 10.1590/s0074-02762003000600019. Epub 2003 Oct 29.
In the present report we analyzed the levels of IgG1, IgG2a, IgG2b and IgG3 isotypes from Balb/c mice immunized with cytoplasmic repetitive antigen (CRA), and flagellar repetitive antigen (FRA) of Trypanosoma cruzi. The immunization was done by subcutaneous route three times (20 days apart) and the analysis was performed 14 days after each treatment. CRA-immunized mice produced high levels of all IgG isotypes, mainly IgG3 and IgG1. FRA-immunization elicited only high levels of IgG1.
在本报告中，我们分析了用克氏锥虫胞质重复抗原（CRA）和鞭毛重复抗原（FRA）免疫的Balb/c小鼠的IgG1、IgG2a、IgG2b和IgG3同种型水平。通过皮下途径进行三次免疫（间隔20天），并在每次治疗后14天进行分析。用CRA免疫的小鼠产生了所有IgG同种型的高水平，主要是IgG3和IgG1。用FRA免疫仅引发了IgG1的高水平。
