Dorsey Cynthia M, Lukas Scott E, Moore Constance M, Tartarini Wendy L, Parow Aimee M, Villafuerte Rosemond A, Renshaw Perry F
Sleep Disorders Center, Behavioral Psychopharmacology Research Laboratory, McLean Hospital, Harvard Medical School, Belmont 02478, USA.
Sleep. 2003 Aug 1;26(5):573-7. doi: 10.1093/sleep/26.5.573.
To investigate chemical changes in the brains of healthy adults after sleep deprivation and recovery sleep, using phosphorous magnetic resonance spectroscopy.
Three consecutive nights (baseline, sleep deprivation, recovery) were spent in the laboratory. Objective sleep measures were assessed on the baseline and recovery nights using polysomnography. Phosphorous magnetic resonance spectroscopy scans took place beginning at 7 am to 8 am on the morning after each of the 3 nights.
Sleep laboratory in a private psychiatric teaching hospital.
Eleven healthy young men.
Following a baseline night of sleep, subjects underwent a night of total sleep deprivation, which involved supervision to ensure the absence of sleep but was not polysomnographically monitored.
No significant changes in any measure of brain chemistry were observed the morning after a night of total sleep deprivation. However, after the recovery night, significant increases in total and beta-nucleoside triphosphate and decreases in phospholipid catabolism, measured by an increase in the concentration of glycerylphosphorylcholine, were observed. Chemical changes paralleled some changes in objective sleep measures.
Significant chemical changes in the brain were observed following recovery sleep after 1 night of total sleep deprivation. The specific process underlying these changes is unclear due to the large brain region sampled in this exploratory study, but changes may reflect sleep inertia or some aspect of the homeostatic sleep mechanism that underlies the depletion and restoration of sleep. Phosphorous magnetic resonance spectroscopy is a technique that may be of value in further exploration of such sleep-wake functions.
使用磷磁共振波谱技术，研究健康成年人在睡眠剥夺及恢复睡眠后大脑中的化学变化。
在实验室度过连续三个夜晚（基线期、睡眠剥夺期、恢复期）。在基线期和恢复期夜晚使用多导睡眠监测仪评估客观睡眠指标。在三个夜晚中的每一晚后的早晨7点至8点进行磷磁共振波谱扫描。
一家私立精神病教学医院的睡眠实验室。
11名健康年轻男性。
在经历一晚基线睡眠后，受试者接受一晚的完全睡眠剥夺，期间进行监督以确保无睡眠，但未进行多导睡眠监测。
在完全睡眠剥夺一晚后的早晨，未观察到任何脑化学指标的显著变化。然而，在恢复睡眠一晚后，观察到总核苷三磷酸和β-核苷三磷酸显著增加，磷脂分解代谢减少，这通过甘油磷酸胆碱浓度的增加来衡量。化学变化与客观睡眠指标的一些变化平行。
在完全睡眠剥夺一晚后的恢复睡眠后，观察到大脑中有显著的化学变化。由于本探索性研究中采样的脑区较大，这些变化背后的具体过程尚不清楚，但这些变化可能反映了睡眠惯性或稳态睡眠机制的某些方面，而稳态睡眠机制是睡眠耗竭和恢复的基础。磷磁共振波谱技术可能在进一步探索此类睡眠-觉醒功能方面具有价值。
