Hoyle D V, Taylor D W
Centre for Tropical Veterinary Medicine, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, EH25 9RG, UK.
Parasite Immunol. 2003 Apr;25(4):221-9. doi: 10.1046/j.1365-3024.2003.00627.x.
In this study we examined regional immune responses to Fasciola hepatica infection in the natural ruminant host. Naïve cattle and those pre-exposed to a drug-abbreviated infection were subsequently challenged and lymph nodes extracted at slaughter. In vitro proliferation and cytokine production by mononuclear cells isolated from hepatic and mesenteric lymph nodes were measured after culture with whole fluke antigen (WFA). Hepatic lymph node cells had a significantly greater response to parasite antigen than mesenteric lymph node cells (P < 0.02), although there was no difference in the magnitude of the proliferative response between naïve and pre-exposed challenged cattle. Mononuclear cells from hepatic lymph nodes produced interferon gamma, interleukin 2 and interleukin 4 after culture with parasite antigen, indicative of a mixed, T helper type 0, response. Comparison of the hepatic node response to a variety of F. hepatica antigens showed that proliferation was lower after culture with cathepsin-L, than with a high molecular weight fraction, WFA or excretory-secretory antigen. Cell culture supernatant fluid from unstimulated hepatic lymph node cells showed an IgG1 response to antigens of 48, 52-70, 82, 96 and 120-190 kDa on Western blot in pre-exposed, but not naïve, challenged animals.
在本研究中，我们检测了天然反刍动物宿主对肝片吸虫感染的局部免疫反应。将未感染过的牛和预先经历过药物缩短疗程感染的牛随后进行攻毒，并在屠宰时提取淋巴结。用全虫抗原（WFA）培养后，测量从肝淋巴结和肠系膜淋巴结分离的单核细胞的体外增殖和细胞因子产生情况。肝淋巴结细胞对寄生虫抗原的反应明显大于肠系膜淋巴结细胞（P < 0.02），尽管在未感染过和预先暴露后攻毒的牛之间增殖反应的幅度没有差异。肝淋巴结的单核细胞在与寄生虫抗原培养后产生干扰素γ、白细胞介素2和白细胞介素4，表明是一种混合的0型辅助性T细胞反应。对肝淋巴结对多种肝片吸虫抗原的反应进行比较表明，与组织蛋白酶-L培养后的增殖低于与高分子量组分、WFA或排泄-分泌抗原培养后的增殖。在预先暴露但未感染过的攻毒动物中，未刺激的肝淋巴结细胞的细胞培养上清液在蛋白质印迹中显示对48、52 - 70、82、96和120 - 190 kDa的抗原产生IgG1反应。
