Oldham G, Williams L
Parasite Immunol. 1985 Sep;7(5):503-16. doi: 10.1111/j.1365-3024.1985.tb00095.x.
Cell mediated immunity (CMI) to Fasciola hepatica antigens was detected by lymphocyte proliferation and interleukin-2 (IL-2) production tests in cattle during the first 4 weeks following liver fluke infection. From the fifth week of infection onwards peripheral blood lymphocytes were unresponsive to fluke antigens by these in vitro tests. Investigations into the cause of this unresponsiveness found no evidence to suggest a selective loss of the IL-2 producing lymphocyte sub-population or that macrophages were responsible for the suppression or that antigen responsive cells were being sequestered in the spleen and mesenteric lymph nodes. Tests carried out on culture supernatants demonstrated the production during this unresponsive period of factors capable of suppressing in vitro responses to PHA. Although further tests failed to show antigen specific suppressor factors the presence of MHC restricted suppressor factors could not be ruled out. The early and transient appearance of CMI during F. hepatica infection of cattle indicates that delayed type hypersensitivity is unlikely to be important in protective immunity in cattle.
在肝片吸虫感染后的前4周，通过淋巴细胞增殖和白细胞介素-2（IL-2）产生试验检测了牛对肝片吸虫抗原的细胞介导免疫（CMI）。从感染的第五周起，通过这些体外试验发现外周血淋巴细胞对吸虫抗原无反应。对这种无反应性原因的调查发现，没有证据表明产生IL-2的淋巴细胞亚群有选择性损失，也没有证据表明巨噬细胞负责抑制作用，或者抗原反应性细胞被隔离在脾脏和肠系膜淋巴结中。对培养上清液进行的试验表明，在这个无反应期产生了能够抑制体外对PHA反应的因子。尽管进一步的试验未能显示抗原特异性抑制因子，但不能排除MHC限制性抑制因子的存在。牛感染肝片吸虫期间CMI的早期和短暂出现表明，迟发型超敏反应在牛的保护性免疫中不太可能起重要作用。
