Bull T J, Sidi-Boumedine K, McMinn E J, Stevenson K, Pickup R, Hermon-Taylor J
Department of Surgery, St George's Hospital Medical School, Cranmer Terrace, SW17 0RE, London, UK.
Mol Cell Probes. 2003 Aug;17(4):157-64. doi: 10.1016/s0890-8508(03)00047-1.
Mycobacterial interspersed repetitive units (MIRU) comprise short tandem repeat structures found at multiple loci throughout the Mycobacterium tuberculosis genome and have been used for typing these pathogens. We have identified MIRU at 18 conserved loci throughout the common portions of the Mycobacterium avium subspecies paratuberculosis (MAP) and M. avium subspecies avium (MAA) genomes. Six of these loci were found to differ between MAA and MAP in the number of tandem repeat motifs occurring at each MIRU locus. Locus specific PCR at 4 of these loci segregated MAP into two major groups, which could be differentiated from ovine-pigmented strains of MAP and the MAP vaccine strain 316F. The same PCR differentiated MAA into five MIRU profiles. PCR at either MIRU locus 1 or MIRU locus 4 distinguished between MAP and all other M. avium complex (MAC) tested. PCR at both loci 1 and 4 also distinguished MAP from Mycobacterium intracellulare. MIRU typing may provide an additional simple and rapid procedure for the differentiation between MAP and other MAC.
分枝杆菌散布重复单位（MIRU）由在结核分枝杆菌基因组中多个位点发现的短串联重复结构组成，并已用于对这些病原体进行分型。我们在鸟分枝杆菌副结核亚种（MAP）和鸟分枝杆菌鸟亚种（MAA）基因组的共同部分中的18个保守位点鉴定出了MIRU。发现这些位点中的6个在每个MIRU位点处出现的串联重复基序数量上，MAA和MAP之间存在差异。在其中4个位点进行的位点特异性PCR将MAP分为两个主要组，这两组可以与MAP的绵羊色素沉着菌株和MAP疫苗株316F区分开来。相同的PCR将MAA分为五种MIRU谱型。在MIRU位点1或MIRU位点4进行的PCR区分了MAP与所有其他测试的鸟分枝杆菌复合群（MAC）。在位点1和4进行的PCR也区分了MAP与胞内分枝杆菌。MIRU分型可能为区分MAP和其他MAC提供另一种简单快速的方法。
