磷脂酰胆碱和二半乳糖基二酰基甘油的干燥及复水后的纯质和混合双层膜中的分子间相互作用：傅里叶变换红外光谱研究
Intermolecular interactions in dry and rehydrated pure and mixed bilayers of phosphatidylcholine and digalactosyldiacylglycerol: a Fourier transform infrared spectroscopy study.
作者信息
Popova Antoaneta V, Hincha Dirk K
机构信息
Max-Planck-Institut für Molekulare Pflanzenphysiologie, D-14424 Potsdam, Germany.
出版信息
Biophys J. 2003 Sep;85(3):1682-90. doi: 10.1016/S0006-3495(03)74598-6.
Abstract
Glycolipids are an important part of almost all biological membranes. Their effects on membrane structure and their interactions with phospholipids, however, have not been extensively studied so far. We have investigated the phase behavior and intermolecular interactions in dry and rehydrated bilayers made from the phospholipid egg phosphatidylcholine (EPC) and the plant chloroplast glycolipid digalactosyldiacylglycerol (DGDG), or from a mixture (1:1) of these lipids, using Fourier transform infrared spectroscopy. We show that there are extensive interactions between EPC and DGDG in mixed membranes, and also between DGDG molecules in pure DGDG membranes, involving sugar OH groups and C[double bond]O, P[double bond]O, and choline moieties in dry membranes. These interactions persist to a certain degree even after rehydration. We present evidence that these interactions influence the mixing behavior in phosphatidylcholine/DGDG membranes and also the phase behavior of both EPC/DGDG and pure DGDG membranes in the dry state.
摘要
糖脂是几乎所有生物膜的重要组成部分。然而，它们对膜结构的影响以及与磷脂的相互作用，迄今为止尚未得到广泛研究。我们利用傅里叶变换红外光谱，研究了由磷脂蛋黄卵磷脂（EPC）和植物叶绿体糖脂二半乳糖基二酰基甘油（DGDG）制成的干燥和再水化双层膜中的相行为和分子间相互作用，或者由这些脂质的混合物（1:1）制成的双层膜中的相行为和分子间相互作用。我们表明，在混合膜中，EPC和DGDG之间存在广泛的相互作用，在纯DGDG膜中的DGDG分子之间也存在广泛的相互作用，这些相互作用涉及干燥膜中的糖羟基以及C=O、P=O和胆碱部分。即使在再水化后，这些相互作用在一定程度上仍然存在。我们提供的证据表明，这些相互作用影响磷脂酰胆碱/DGDG膜中的混合行为，以及EPC/DGDG和纯DGDG膜在干燥状态下的相行为。
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