胆固醇对干双分子层的影响：磷脂酰胆碱不饱和度与糖脂或游离糖之间的相互作用。
Effects of cholesterol on dry bilayers: interactions between phosphatidylcholine unsaturation and glycolipid or free sugar.
作者信息
Popova Antoaneta V, Hincha Dirk K
机构信息
Max-Planck-Institut für Molekulare Pflanzenphysiologie, D-14424 Potsdam, Germany.
出版信息
Biophys J. 2007 Aug 15;93(4):1204-14. doi: 10.1529/biophysj.107.108886. Epub 2007 May 25.
Abstract
Cholesterol and other sterols are important components of biological membranes and are known to strongly influence the physical characteristics of lipid bilayers. Although this has been studied extensively in fully hydrated membranes, little is known about the effects of cholesterol on the stability of membranes in the dry state. Here, we present a Fourier transform infrared spectroscopy study on the effects of cholesterol on the phase behavior of dry liposomes composed of phosphatidylcholines with different degrees of fatty acid unsaturation or of mixtures of phosphatidylcholine with a plant galactolipid. In addition, we have analyzed the H-bonding of cholesterol, galactose, and a combination of the two additives to the P=O and C=O groups in dry phosphatidylcholine bilayers. The data indicate a complex balance of interactions between the different components in the dry state and a strong influence of fatty acid unsaturation on the interactions of the diacyl lipids with both cholesterol and galactose.
摘要
胆固醇和其他甾醇是生物膜的重要组成部分，并且已知它们会强烈影响脂质双层的物理特性。尽管这在完全水合的膜中已得到广泛研究，但关于胆固醇对干燥状态下膜稳定性的影响却知之甚少。在此，我们展示了一项傅里叶变换红外光谱研究，该研究针对胆固醇对由不同脂肪酸不饱和度的磷脂酰胆碱或磷脂酰胆碱与植物半乳糖脂的混合物组成的干燥脂质体的相行为的影响。此外，我们分析了干燥磷脂酰胆碱双层中胆固醇、半乳糖以及这两种添加剂组合与P=O和C=O基团之间的氢键作用。数据表明干燥状态下不同组分之间存在复杂的相互作用平衡，并且脂肪酸不饱和度对二酰基脂质与胆固醇和半乳糖的相互作用有强烈影响。
相似文献
1
Effects of cholesterol on dry bilayers: interactions between phosphatidylcholine unsaturation and glycolipid or free sugar.胆固醇对干双分子层的影响：磷脂酰胆碱不饱和度与糖脂或游离糖之间的相互作用。
Biophys J. 2007 Aug 15;93(4):1204-14. doi: 10.1529/biophysj.107.108886. Epub 2007 May 25.
2
Effects of the sugar headgroup of a glycoglycerolipid on the phase behavior of phospholipid model membranes in the dry state.糖基甘油酯的糖头部基团对干燥状态下磷脂模型膜相行为的影响。
Glycobiology. 2005 Nov;15(11):1150-5. doi: 10.1093/glycob/cwj001. Epub 2005 Jun 22.
3
Intermolecular interactions in dry and rehydrated pure and mixed bilayers of phosphatidylcholine and digalactosyldiacylglycerol: a Fourier transform infrared spectroscopy study.磷脂酰胆碱和二半乳糖基二酰基甘油的干燥及复水后的纯质和混合双层膜中的分子间相互作用：傅里叶变换红外光谱研究
Biophys J. 2003 Sep;85(3):1682-90. doi: 10.1016/S0006-3495(03)74598-6.
4
Specific interactions of tryptophan with phosphatidylcholine and digalactosyldiacylglycerol in pure and mixed bilayers in the dry and hydrated state.色氨酸在干燥和水合状态下的纯双层及混合双层中与磷脂酰胆碱和二半乳糖基二酰基甘油的特异性相互作用。
Chem Phys Lipids. 2004 Dec;132(2):171-84. doi: 10.1016/j.chemphyslip.2004.06.003.
5
Glycolipid membranes through atomistic simulations: effect of glucose and galactose head groups on lipid bilayer properties.通过原子模拟研究糖脂膜：葡萄糖和半乳糖头部基团对脂质双层性质的影响。
J Phys Chem B. 2007 Aug 30;111(34):10146-54. doi: 10.1021/jp0730895. Epub 2007 Aug 3.
6
Components of the carbonyl stretching band in the infrared spectra of hydrated 1,2-diacylglycerolipid bilayers: a reevaluation.水合1,2 - 二酰基甘油脂质双层红外光谱中羰基伸缩带的组成部分：重新评估
Biophys J. 1994 Dec;67(6):2367-75. doi: 10.1016/S0006-3495(94)80723-4.
7
Time-resolved fluorescence and fourier transform infrared spectroscopic investigations of lateral packing defects and superlattice domains in compositionally uniform cholesterol/phosphatidylcholine bilayers.对组成均匀的胆固醇/磷脂酰胆碱双层膜中侧向堆积缺陷和超晶格域的时间分辨荧光和傅里叶变换红外光谱研究。
Biophys J. 2003 Jun;84(6):3777-91. doi: 10.1016/S0006-3495(03)75106-6.
8
Dehydrating phospholipid vesicles measured in real-time using ATR Fourier transform infrared spectroscopy.实时测量脱水磷脂囊泡的衰减全反射傅里叶变换红外光谱。
Cryobiology. 2010 Aug;61(1):108-14. doi: 10.1016/j.cryobiol.2010.06.001. Epub 2010 Jun 8.
9
A 2H solid-state NMR spectroscopic investigation of biomimetic bicelles containing cholesterol and polyunsaturated phosphatidylcholine.对含有胆固醇和多不饱和磷脂酰胆碱的仿生双分子层进行的2H固态核磁共振光谱研究。
Chem Phys Lipids. 2004 Nov;132(1):55-64. doi: 10.1016/j.chemphyslip.2004.09.005.
10
Comparison of ternary bilayer mixtures with asymmetric or symmetric unsaturated phosphatidylcholine lipids by coarse grained molecular dynamics simulations.采用粗粒化分子动力学模拟比较具有不对称或对称不饱和磷脂酰胆碱脂质的三元双层混合物。
J Phys Chem B. 2012 Mar 22;116(11):3525-37. doi: 10.1021/jp212406u. Epub 2012 Mar 9.
引用本文的文献
1
Antitumor Assessment of Liposomal Beta-Carotene with Tamoxifen Against Breast Carcinoma Cell Line: An In Vitro Study.脂质体β-胡萝卜素联合他莫昔芬对乳腺癌细胞系的抗肿瘤评估：一项体外研究。
Biomolecules. 2025 Mar 26;15(4):486. doi: 10.3390/biom15040486.
2
Innovative Approaches to Enhancing the Biomedical Properties of Liposomes.增强脂质体生物医学特性的创新方法。
Pharmaceutics. 2024 Nov 27;16(12):1525. doi: 10.3390/pharmaceutics16121525.
3
Modifying Membranotropic Action of Antimicrobial Peptide Gramicidin S by Star-like Polyacrylamide and Lipid Composition of Nanocontainers.通过星型聚丙烯酰胺和纳米容器的脂质组成来修饰抗菌肽短杆菌素 S 的膜转导活性。
Int J Mol Sci. 2024 Aug 9;25(16):8691. doi: 10.3390/ijms25168691.
4
Nanoplatform Based Intranasal Vaccines: Current Progress and Clinical Challenges.基于纳米平台的鼻腔内疫苗：当前进展和临床挑战。
ACS Nano. 2024 Sep 10;18(36):24650-24681. doi: 10.1021/acsnano.3c10797. Epub 2024 Aug 26.
5
Magnetic-Responsive Liposomal Hydrogel Membranes for Controlled Release of Small Bioactive Molecules-An Insight into the Release Kinetics.用于小生物活性分子控释的磁响应脂质体水凝胶膜——对释放动力学的深入研究
Membranes (Basel). 2023 Jul 17;13(7):674. doi: 10.3390/membranes13070674.
6
Development and Optimisation of Inhalable EGCG Nano-Liposomes as a Potential Treatment for Pulmonary Arterial Hypertension by Implementation of the Design of Experiments Approach.通过实验设计方法开发和优化可吸入表没食子儿没食子酸酯纳米脂质体作为肺动脉高压的潜在治疗方法
Pharmaceutics. 2023 Feb 6;15(2):539. doi: 10.3390/pharmaceutics15020539.
7
Liposome-polymer complex for drug delivery system and vaccine stabilization.用于药物递送系统和疫苗稳定化的脂质体-聚合物复合物
Heliyon. 2022 Feb 12;8(2):e08934. doi: 10.1016/j.heliyon.2022.e08934. eCollection 2022 Feb.
8
Structural properties and enzyme stabilization function of the intrinsically disordered LEA_4 protein TdLEA3 from wheat.小麦内在无序 LEA_4 蛋白 TdLEA3 的结构特性和酶稳定功能。
Sci Rep. 2019 Mar 6;9(1):3720. doi: 10.1038/s41598-019-39823-w.
9
Lyophilization of Liposomal Formulations: Still Necessary, Still Challenging.脂质体制剂的冻干：仍有必要，仍具挑战。
Pharmaceutics. 2018 Aug 28;10(3):139. doi: 10.3390/pharmaceutics10030139.
10
Polyprenols Are Synthesized by a Plastidial -Prenyltransferase and Influence Photosynthetic Performance.聚戊烯醇由质体异戊烯基转移酶合成并影响光合性能。
Plant Cell. 2017 Jul;29(7):1709-1725. doi: 10.1105/tpc.16.00796. Epub 2017 Jun 27.
本文引用的文献
1
A molecular view of melting in anhydrous phospholipidic membranes.无水磷脂膜中熔化的分子观点。
Biophys J. 2007 Jan 1;92(1):147-61. doi: 10.1529/biophysj.106.089631.
2
Domain-formation in DOPC/SM bilayers studied by pfg-NMR: effect of sterol structure.通过脉冲场梯度核磁共振研究二油酰磷脂酰胆碱/鞘磷脂双层膜中的结构域形成：甾醇结构的影响
Biophys J. 2006 Oct 1;91(7):2501-7. doi: 10.1529/biophysj.106.085480. Epub 2006 Jul 7.
3
Phase diagram of stigmasterol-dipalmitoylphosphatidylcholine mixtures dispersed in excess water.分散于过量水中的豆甾醇-二棕榈酰磷脂酰胆碱混合物的相图。
Biochim Biophys Acta. 2006 Jun;1758(6):764-71. doi: 10.1016/j.bbamem.2006.04.017. Epub 2006 May 5.
4
Monosaccharide composition, chain length and linkage type influence the interactions of oligosaccharides with dry phosphatidylcholine membranes.单糖组成、链长和连接类型会影响寡糖与干燥磷脂酰胆碱膜的相互作用。
Biochim Biophys Acta. 2006 May;1758(5):680-91. doi: 10.1016/j.bbamem.2006.04.005. Epub 2006 Apr 21.
5
Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles.海藻糖对二棕榈酰磷脂酰胆碱-胆固醇单层囊泡相行为的影响。
Biochim Biophys Acta. 2006 Jan;1758(1):65-73. doi: 10.1016/j.bbamem.2006.01.002. Epub 2006 Jan 27.
6
Low amounts of sucrose are sufficient to depress the phase transition temperature of dry phosphatidylcholine, but not for lyoprotection of liposomes.低量的蔗糖足以降低干燥磷脂酰胆碱的相变温度，但对脂质体的冻干保护而言并不足够。
Biophys J. 2006 Apr 15;90(8):2831-42. doi: 10.1529/biophysj.105.074427. Epub 2006 Jan 27.
7
Modulating membrane properties: the effect of trehalose and cholesterol on a phospholipid bilayer.调节膜特性：海藻糖和胆固醇对磷脂双层的影响。
J Phys Chem B. 2005 Dec 22;109(50):24173-81. doi: 10.1021/jp054843u.
8
Effects of the sugar headgroup of a glycoglycerolipid on the phase behavior of phospholipid model membranes in the dry state.糖基甘油酯的糖头部基团对干燥状态下磷脂模型膜相行为的影响。
Glycobiology. 2005 Nov;15(11):1150-5. doi: 10.1093/glycob/cwj001. Epub 2005 Jun 22.
9
Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose.用海藻糖稳定的冻干磷脂 - 胆固醇混合物的相行为
Biochim Biophys Acta. 2005 Jul 15;1713(1):57-64. doi: 10.1016/j.bbamem.2005.05.001.
10
Specific interactions of tryptophan with phosphatidylcholine and digalactosyldiacylglycerol in pure and mixed bilayers in the dry and hydrated state.色氨酸在干燥和水合状态下的纯双层及混合双层中与磷脂酰胆碱和二半乳糖基二酰基甘油的特异性相互作用。
Chem Phys Lipids. 2004 Dec;132(2):171-84. doi: 10.1016/j.chemphyslip.2004.06.003.
Zotero 插件