嗜酒栖热菌的Na⁺转运F₀F₁ - ATP合酶在ATP水解和合成过程中的电荷位移
Charge displacements during ATP-hydrolysis and synthesis of the Na+-transporting FoF1-ATPase of Ilyobacter tartaricus.
作者信息
Burzik Christiane, Kaim Georg, Dimroth Peter, Bamberg Ernst, Fendler Klaus
机构信息
Max-Planck-Institut für Biophysik, D-60596 Frankfurt/Main, Germany.
出版信息
Biophys J. 2003 Sep;85(3):2044-54. doi: 10.1016/S0006-3495(03)74632-3.
Abstract
Transient electrical currents generated by the Na(+)-transporting F(o)F(1)-ATPase of Ilyobacter tartaricus were observed in the hydrolytic and synthetic mode of the enzyme. Two techniques were applied: a photochemical ATP concentration jump on a planar lipid membrane and a rapid solution exchange on a solid supported membrane. We have identified an electrogenic reaction in the reaction cycle of the F(o)F(1)-ATPase that is related to the translocation of the cation through the membrane bound F(o) subcomplex of the ATPase. In addition, we have determined rate constants for the process: For ATP hydrolysis this reaction has a rate constant of 15-30 s(-1) if H(+) is transported and 30-60 s(-1) if Na(+) is transported. For ATP synthesis the rate constant is 50-70 s(-1).
摘要
在发酵乳杆菌的Na(+)-转运F(o)F(1)-ATP酶的水解模式和合成模式中观察到了瞬态电流。应用了两种技术：平面脂质膜上的光化学ATP浓度跃变和固体支持膜上的快速溶液交换。我们在F(o)F(1)-ATP酶的反应循环中确定了一种电生反应，该反应与阳离子通过ATP酶的膜结合F(o)亚复合体的转运有关。此外，我们还确定了该过程的速率常数：对于ATP水解，如果转运H(+)，该反应的速率常数为15 - 30 s(-1)，如果转运Na(+)，则为30 - 60 s(-1)。对于ATP合成，速率常数为50 - 70 s(-1)。
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本文引用的文献
1
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J Bioenerg Biomembr. 2000 Oct;32(5):517-21. doi: 10.1023/a:1005673209883.
2
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FEBS Lett. 2002 Sep 11;527(1-3):147-52. doi: 10.1016/s0014-5793(02)03198-8.
3
Coupled rotation within single F0F1 enzyme complexes during ATP synthesis or hydrolysis.在ATP合成或水解过程中，单个F0F1酶复合物内的耦合旋转。
FEBS Lett. 2002 Aug 14;525(1-3):156-63. doi: 10.1016/s0014-5793(02)03097-1.
4
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Biochemistry. 2001 Nov 13;40(45):13744-52. doi: 10.1021/bi011223k.
5
Bacterial Na(+)-ATP synthase has an undecameric rotor.细菌钠-ATP合酶有一个十一聚体转子。
EMBO Rep. 2001 Mar;2(3):229-33. doi: 10.1093/embo-reports/kve047.
6
Rotation of the c subunit oligomer in fully functional F1Fo ATP synthase.在功能完备的F1Fo ATP合酶中c亚基寡聚体的旋转
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7
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