Kingo K, Kõks S, Silm H, Vasar E
Department of Dermatology and Venerology, University of Tartu, Tartu, Estonia.
Genes Immun. 2003 Sep;4(6):455-7. doi: 10.1038/sj.gene.6364004.
We analyzed three single-nucleotide polymorphisms (SNPs) at the interleukin-10 (IL-10) 5' flanking region (positions -1082 A/G, -819 C/T and -592 C/A) in an association case-control study involving 248 patients with plaque type of psoriasis and 148 unrelated healthy volunteers using ARMS (amplification refractory mutation system)-PCR (Polymerase Chain Reaction) method. No difference was found in the frequencies of haplotype distribution between healthy controls and patients with psoriasis. There were no significant differences in the IL-10 haplotype distribution depending on the age of onset and family history of psoriasis. However, the results of our study demonstrate that the IL-10 haplotype has a role in determining severity and course of plaque type of psoriasis. IL-10 ACC haplotype (P<0.05) is likely to be defining lower activity of disease (PASI</=20; extent</=10%) and ATA haplotype is likely to be associated with persistent eruption (P<0.01). As ACC haplotype is suggested to be associated with high IL-10 secretion and ATA is related to low IL-10 secretion, potential differences in the IL-10 secretion levels might contribute the differences in the clinical course of psoriasis.
我们在一项病例对照研究中，采用扩增阻滞突变系统（ARMS）-聚合酶链反应（PCR）方法，分析了白细胞介素-10（IL-10）5'侧翼区的三个单核苷酸多态性（SNP）（位置-1082 A/G、-819 C/T和-592 C/A），该研究纳入了248例斑块型银屑病患者和148名无亲缘关系的健康志愿者。在健康对照组和银屑病患者之间，未发现单倍型分布频率存在差异。根据银屑病的发病年龄和家族史，IL-10单倍型分布也无显著差异。然而，我们的研究结果表明，IL-10单倍型在决定斑块型银屑病的严重程度和病程方面发挥作用。IL-10的ACC单倍型（P<0.05）可能与较低的疾病活动度（银屑病面积和严重程度指数[PASI]≤20；累及范围≤10%）相关，而ATA单倍型可能与持续性皮疹相关（P<0.01）。由于ACC单倍型被认为与高IL-10分泌相关，而ATA与低IL-10分泌相关，因此IL-10分泌水平的潜在差异可能导致银屑病临床病程的差异。
