Marczak Ewa D, Usui Hachiro, Fujita Hiroyuki, Yang Yanjun, Yokoo Megumi, Lipkowski Andrzej W, Yoshikawa Masaaki
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
Peptides. 2003 Jun;24(6):791-8. doi: 10.1016/s0196-9781(03)00174-8.
Four potent angiotensin converting enzyme (ACE) inhibitory peptides, IY, RIY, VW and VWIS, were isolated from subtilisin digest of rapeseed protein. Among them RIY and VWIS are new peptides with IC(50) 28 and 30 microM, respectively. All isolated peptides lowered blood pressure of spontaneously hypertensive rats (SHR) following oral administration. The maximum effect in the case of RIY was observed 4h after administration, while maximum effect of other peptides on blood pressure occurred 2h after administration. Furthermore, the antihypertensive effect of RIY was observed even in old rats, in which ACE inhibitors become less effective, suggesting that a different mechanism other than ACE inhibition is also involved in lowering blood pressure by this peptide. Subtilisin digest of rapeseed protein also significantly lowered blood pressure of SHR after oral administration of a single dosage 0.15 g/kg, exerting maximum antihypertensive effect 4h after administration. This digest appears promising as a functional food, which may be useful in the prevention and treatment of hypertension.
从菜籽蛋白的枯草杆菌蛋白酶消化物中分离出四种有效的血管紧张素转换酶（ACE）抑制肽，即IY、RIY、VW和VWIS。其中，RIY和VWIS是新肽，其IC(50)分别为28和30微摩尔。所有分离出的肽经口服后均可降低自发性高血压大鼠（SHR）的血压。RIY在给药后4小时观察到最大效果，而其他肽对血压的最大效果在给药后2小时出现。此外，即使在ACE抑制剂效果较差的老年大鼠中也观察到了RIY的降压作用，这表明该肽降低血压的机制除了ACE抑制外，还涉及其他不同的机制。菜籽蛋白的枯草杆菌蛋白酶消化物在口服单剂量0.15克/千克后也显著降低了SHR的血压，在给药后4小时发挥最大降压作用。这种消化物作为一种功能性食品似乎很有前景，可能对高血压的预防和治疗有用。
