Nakamura Y, Yamamoto N, Sakai K, Takano T
Research and Development Center, Calpis Food Industry Co., Ltd., Kanagawa, Japan.
J Dairy Sci. 1995 Jun;78(6):1253-7. doi: 10.3168/jds.S0022-0302(95)76745-5.
This study reports the antihypertensive effect of orally administered doses of either Calpis sour milk or peptides (Val-Pro-Pro and Ile-Pro-Pro), which are inhibitors to angiotensin I-converting enzyme, isolated from the sour milk using strain SHR spontaneously hypertensive rats. Single oral administration of the sour milk (5 ml/kg of BW), corresponding inhibitory units of the peptides Val-Pro-Pro (.6 mg/kg of BW), or Ile-Pro-Pro (.3 mg/kg of BW) significantly decreased the systolic blood pressure from 6 to 8 h after administration. Blood pressure returned to the initial level at 24 h after administration. Antihypertensive activity of these two tripeptides was dose-dependent up to 5 mg/kg of BW. Conversely, the sour milk (25 ml/kg of BW) and mixed tripeptides (10 mg each of Val-Pro-Pro and Ile-Pro-Pro/kg of BW) did not change the systolic blood pressure of the normotensive strain WKY Wistar-Kyoto rats.
本研究报告了口服卡尔皮斯酸牛奶或从酸牛奶中分离出的血管紧张素I转换酶抑制剂肽（缬氨酸-脯氨酸-脯氨酸和异亮氨酸-脯氨酸-脯氨酸）对自发性高血压大鼠（SHR）的降压作用。单次口服酸牛奶（5毫升/千克体重）、肽缬氨酸-脯氨酸-脯氨酸相应抑制单位（0.6毫克/千克体重）或异亮氨酸-脯氨酸-脯氨酸（0.3毫克/千克体重），给药后6至8小时收缩压显著降低。给药后24小时血压恢复到初始水平。这两种三肽的降压活性在高达5毫克/千克体重时呈剂量依赖性。相反，酸牛奶（25毫升/千克体重）和混合三肽（缬氨酸-脯氨酸-脯氨酸和异亮氨酸-脯氨酸-脯氨酸各10毫克/千克体重）对正常血压的WKY大鼠（Wistar-Kyoto大鼠）的收缩压没有影响。
