Department of Botany, PMAS Arid Agriculture University, Rawalpindi 46300, Pakistan.
Department of Chemistry, Hazara University, Mansehra, Pakistan.
IET Nanobiotechnol. 2019 Feb;13(1):46-51. doi: 10.1049/iet-nbt.2018.5012.
With the progression of nanotechnology, the use of nanoparticles (NPs) in consumer products has increased dramatically and green synthesis is one of the cheapest and eco-friendly methods to obtain non-hazardous NPs. In the current research zinc (Zn) NPs synthesis was carried out by using the fresh and healthy leaves of L. followed by characterisation through ultraviolet (UV)-visible spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM). UV-visible spectroscopy confirmed the green synthesis of ZnNPs, while XRD confirmed the size of NPs, which was 30-70 nm. SEM shows that the shape of ZnNPs was irregular. The effects of green synthesised NPs on two different varieties of were evaluated. Exposure to ZnNPs (5, 15, and 25 mg/l) caused a significant increase in root and shoot length of The application of NPs significantly improved plant germination and triggered the production of secondary metabolite and antioxidant enzymes. ZnNPs showed a significant increase in chlorophyll, superoxide dismutase, total flavonoid content (TFC) and antioxidant enzymes while total phenolic content was decreased when TFC increased. Thus, it has been concluded from the current study that ZnNPs may possibly trigger the production of antioxidant enzymes and various biochemical compounds.
随着纳米技术的发展，纳米粒子（NPs）在消费产品中的应用急剧增加，绿色合成是获得非危险 NPs 的最便宜和最环保的方法之一。在当前的研究中，使用新鲜健康的 L. 叶片进行了锌（Zn）NPs 的合成，然后通过紫外（UV）-可见光谱、X 射线衍射（XRD）和扫描电子显微镜（SEM）进行了表征。紫外-可见光谱证实了 ZnNPs 的绿色合成，而 XRD 证实了 NPs 的尺寸，为 30-70nm。SEM 显示 ZnNPs 的形状不规则。评价了绿色合成 NPs 对两种不同品种 的影响。暴露于 ZnNPs（5、15 和 25mg/l）会导致 的根和茎长度显著增加。NPs 的应用显著提高了植物的发芽率，并触发了次生代谢产物和抗氧化酶的产生。ZnNPs 显著增加了叶绿素、超氧化物歧化酶、总类黄酮含量（TFC）和抗氧化酶，而 TFC 增加时总酚含量降低。因此，从当前的研究中可以得出结论，ZnNPs 可能触发抗氧化酶和各种生化化合物的产生。
