控制日本慢生根瘤菌中共生基因表达的两个调控级联对氧气的反应不同。
Disparate oxygen responsiveness of two regulatory cascades that control expression of symbiotic genes in Bradyrhizobium japonicum.
作者信息
Sciotti Michel-Angelo, Chanfon Astrid, Hennecke Hauke, Fischer Hans-Martin
机构信息
Institut für Mikrobiologie, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland.
出版信息
J Bacteriol. 2003 Sep;185(18):5639-42. doi: 10.1128/JB.185.18.5639-5642.2003.
Abstract
Two oxygen-responsive regulatory systems controlling numerous symbiotic genes in Bradyrhizobium japonicum were assayed in free-living cultures for their capacity to activate target genes under different oxygen conditions. NifA- and FixLJ-controlled target genes showed disparate relative expression patterns. Induction of NifA-dependent genes was observed only at oxygen concentrations below 2% in the gas phase, whereas that of FixLJ-controlled targets progressively increased when the oxygen concentration was lowered from 21 to 5, 2, or 0.5%. We propose that this reflects a response to a gradient of increasing oxygen deprivation as bacteria invade their host during root nodule development.
摘要
在自由生活培养物中检测了控制日本慢生根瘤菌中众多共生基因的两个氧响应调节系统，以评估它们在不同氧条件下激活靶基因的能力。由NifA和FixLJ控制的靶基因表现出不同的相对表达模式。仅在气相氧浓度低于2%时观察到NifA依赖性基因的诱导，而当氧浓度从21%降至5%、2%或0.5%时，FixLJ控制的靶基因的诱导则逐渐增加。我们认为，这反映了在根瘤发育过程中细菌侵入宿主时对氧剥夺增加梯度的一种反应。
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