Poletti G, Orsini F, Lenardi C, Barborini E
Istituto di Fisiologia Generale e Chimica Biologica, Università di Milano, Milan, Italy.
J Microsc. 2003 Sep;211(Pt 3):249-55. doi: 10.1046/j.1365-2818.2003.01220.x.
A comparative study of AFM and SEM imaging of the same area of a human scalp hair has been carried out to determine the similarity and the differences between the two techniques. Sample preparation for SEM analysis requires a metallization step and vacuum exposure, both of which could potentially induce modifications to the surface details. By contrast, AFM is a suitable technique to evaluate any effect resulting from sample manipulation because it can be applied without any specific treatment. AFM analysis demonstrates that sample metallization is responsible for modifications to the surface details of hair, mainly comprising an increase in height of scale steps and of root mean square roughness together with variation in scale profiles. Sample treatments for SEM imaging are in general potentially responsible for surface modifications to the samples involved.
对人类头皮同一区域毛发进行了原子力显微镜（AFM）和扫描电子显微镜（SEM）成像的对比研究，以确定这两种技术之间的异同。SEM分析的样品制备需要金属化步骤和真空暴露，这两者都可能对表面细节产生潜在的改变。相比之下，AFM是评估样品处理产生的任何影响的合适技术，因为它无需任何特殊处理即可应用。AFM分析表明，样品金属化导致毛发表面细节发生改变，主要包括鳞片台阶高度和均方根粗糙度增加以及鳞片轮廓变化。一般来说，SEM成像的样品处理可能会对所涉及的样品表面产生改变。
