Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China.
Nanoscale Res Lett. 2011 Jul 16;6(1):455. doi: 10.1186/1556-276X-6-455.
A comparative study of immature and mature bone marrow-derived dendritic cells (BMDCs) was first performed through an atomic force microscope (AFM) to clarify differences of their nanostructure and adhesion force. AFM images revealed that the immature BMDCs treated by granulocyte macrophage-colony stimulating factor plus IL-4 mainly appeared round with smooth surface, whereas the mature BMDCs induced by lipopolysaccharide displayed an irregular shape with numerous pseudopodia or lamellapodia and ruffles on the cell membrane besides becoming larger, flatter, and longer. AFM quantitative analysis further showed that the surface roughness of the mature BMDCs greatly increased and that the adhesion force of them was fourfold more than that of the immature BMDCs. The nano-features of the mature BMDCs were supported by a high level of IL-12 produced from the mature BMDCs and high expression of MHC-II on the surface of them. These findings provide a new insight into the nanostructure of the immature and mature BMDCs.
首先通过原子力显微镜（AFM）对未成熟和成熟骨髓来源树突状细胞（BMDC）进行比较研究，以阐明它们纳米结构和粘附力的差异。AFM 图像显示，经粒细胞巨噬细胞集落刺激因子加白细胞介素-4 处理的未成熟 BMDC 主要呈圆形，表面光滑，而经脂多糖诱导的成熟 BMDC 则呈现不规则形状，细胞膜上有许多伪足或片状伪足和皱褶，并且变得更大、更平、更长。AFM 定量分析进一步表明，成熟 BMDC 的表面粗糙度大大增加，其粘附力是未成熟 BMDC 的四倍。成熟 BMDC 具有较高的纳米特征，这是由成熟 BMDC 产生的高水平白细胞介素-12 和表面高表达 MHC-II 支持的。这些发现为未成熟和成熟 BMDC 的纳米结构提供了新的见解。
