Gupta A K, Ahmad I, Porretta M, Summerbell R C
Division of Dermatology, Department of Medicine, Sunnybrook and Women's College Health Sciences Center (Sunnybrook site), and the University of Toronto, Toronto, Ontario, Canada N6K 1L6.
Mycoses. 2003 Sep;46(8):322-8. doi: 10.1046/j.1439-0507.2003.00879.x.
Arthroconidia produced by dermatophytic fungi are considered to be the primary cause of skin and nail infections in humans and animals. Trichophyton rubrum is currently the most common cause of tinea pedis all over the world. The common form of T. rubrum produces a cottony colony in cultures that is characteristically low in conidia formation. The attempts to produce arthroconidia in T. rubrum have shown little success so far. Recently, Trichophyton raubitschekii, an anthropophilic dermatophyte prevalent in Asia, Africa, and the Mediterranean, has been recognized as a variant of T. rubrum. In cultures, T. raubitschekii is characterized by a granular colony form, and an abundance of both micro- and macroconidia. The present study reveals a predominance of arthroconidia in two T. raubitschekii cultures isolated from clinical materials. These isolates were able to maintain arthroconidiation in bimonthly subcultures throughout the entire course of this study. The growth parameters for in vitro cultivation of arthroconidia are described here. Arthroconidia prepared from T. raubitschekii cultures showed greater than 95% germination within 21 h of suspension in phosphate-buffered saline. The availability of arthroconidia in T. raubitschekii cultures appears to offer a practical means of characterizing infective cells in T. rubrum.
皮肤癣菌产生的关节孢子被认为是人和动物皮肤及指甲感染的主要原因。红色毛癣菌是目前全球足癣最常见的病因。红色毛癣菌的常见形态在培养物中形成棉絮状菌落，其分生孢子形成能力通常较低。到目前为止，在红色毛癣菌中产生关节孢子的尝试收效甚微。最近，劳氏毛癣菌，一种在亚洲、非洲和地中海地区流行的嗜人皮肤癣菌，已被确认为红色毛癣菌的一个变种。在培养物中，劳氏毛癣菌的特征是呈颗粒状菌落形态，并有大量的小分生孢子和大分生孢子。本研究揭示了从临床材料分离出的两种劳氏毛癣菌培养物中关节孢子占优势。在本研究的整个过程中，这些分离株能够在每两个月一次的传代培养中保持关节孢子形成。本文描述了关节孢子体外培养的生长参数。从劳氏毛癣菌培养物制备的关节孢子在磷酸盐缓冲盐水中悬浮21小时内发芽率大于95%。劳氏毛癣菌培养物中关节孢子的存在似乎为鉴定红色毛癣菌中的感染细胞提供了一种实用方法。
