Hollander Chelsea den, Visser Jasper, de Haas Ellen, Incrocci Luca, Smijs Threes
Department of Radiotherapy, Erasmus Medical Centre, P.O. Box 2040, Office Ee-1683, 3000-CA Rotterdam, The Netherlands.
Department of Dermatology and Venereology, Erasmus Medical Centre, P.O. Box 2040, Office Ee-1683, 3000-CA Rotterdam, The Netherlands.
J Fungi (Basel). 2015 Jul 27;1(2):138-153. doi: 10.3390/jof1020138.
Onychomycosis is predominantly caused by the dermatophytes , and The main treatment obstacle concerns low nail-plate drug permeability. antifungal photodynamic treatment (PDT) and nail penetration enhancing effectiveness have been proven for multifunctional photosensitizer 5,10,15-(4--methylpyridinium)-20-(4-(butyramido-methylcysteinyl)-hydroxyphenyl)-[21,23]-porphine trichloride (PORTHE). This study investigates single PORTHE green laser/LED PDT of varying degrees of onychomycoses in a human nail model. , , onychomycoses were induced on nail pieces at 28 °C (normal air) and 37 °C (6.4% CO₂) during 3 to 35 days and PDTs applied to the 37 °C infections. All dermatophytes showed increasingly nail plate invasion at 37 °C between 7 and 35 days; arthroconidia were observed after 35 days for and . Using 81 J/cm² (532 nm) 7-day onychomycoses were cured (92%) with 80 µM PORTHE (pH 8) after 24 h propylene glycol (PG, 40%) pre-treatment and 35-day onychomycoses (52%-67%) with 24 h PORTHE (40-80 µM)/40% PG treatment (pH 5). 28 J/cm² LED light (525 ± 37 nm) improved cure rates to 72%, 83% and 73% for, respectively, , and 35-day onychomycoses and to 100% after double PDT. Data indicate PDT relevance for onychomycosis.
甲真菌病主要由皮肤癣菌引起，主要治疗障碍在于甲板药物渗透性低。已证实多功能光敏剂5,10,15-(4-甲基吡啶鎓)-20-(4-(丁酰胺甲基半胱氨酰)-羟基苯基)-[21,23]-卟吩三氯化物（PORTHE）的抗真菌光动力疗法（PDT）及增强指甲渗透效果。本研究在人指甲模型中调查不同程度甲真菌病的单次PORTHE绿色激光/LED PDT情况。在28℃（正常空气）和37℃（6.4%二氧化碳）条件下，在指甲片上诱发甲真菌病3至35天，并对37℃感染部位进行PDT治疗。所有皮肤癣菌在37℃条件下7至35天内甲板侵袭逐渐增加；35天后在[具体菌种1]和[具体菌种2]中观察到关节分生孢子。使用81 J/cm²（532 nm），在24小时丙二醇（PG，40%）预处理后，80 µM PORTHE（pH 8）可治愈7天的甲真菌病（92%），24小时PORTHE（40 - 80 µM）/40% PG治疗（pH 5）可治愈35天的甲真菌病（52% - 67%）。28 J/cm² LED光（525 ± 37 nm）分别将7天、14天和35天甲真菌病的治愈率提高到72%、83%和73%，二次PDT后治愈率达100%。数据表明PDT对甲真菌病具有相关性。
