Berry Catherine C, Wells Stephen, Charles Stuart, Curtis Adam S G
Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, Scotland, UK.
Biomaterials. 2003 Nov;24(25):4551-7. doi: 10.1016/s0142-9612(03)00237-0.
Magnetic nanoparticles have been used for biomedical purposes for several years. In recent years, nanotechnology has developed to a stage that makes it possible to engineer particles to provide opportunities for the site-specific delivery of drugs. To this end a variety of iron oxide particles have been synthesised. The size and surface of the particles are crucial factors in the application of the particles. This study therefore involves the use of magnetic nanoparticles synthesised and derivatised with either dextran or albumin, compared to identical underivatised plain particles. This influence in vitro was assessed using human dermal fibroblasts and various techniques to observe cell-particles interaction, including light and fluorescence microscopy, scanning and transmission electron microscopy. The results indicate that the derivatised particles induce alterations in cell behaviour and morphology distinct from the plain particles, suggesting that cell response can be directed via specifically engineered particle surfaces.
磁性纳米颗粒已用于生物医学目的数年。近年来，纳米技术已发展到一个阶段，使得设计颗粒以提供药物定点递送的机会成为可能。为此，已合成了多种氧化铁颗粒。颗粒的大小和表面是颗粒应用中的关键因素。因此，本研究涉及使用用葡聚糖或白蛋白合成并衍生化的磁性纳米颗粒，并与相同的未衍生化普通颗粒进行比较。使用人皮肤成纤维细胞和各种技术评估这种体外影响，以观察细胞与颗粒的相互作用，包括光学和荧光显微镜、扫描和透射电子显微镜。结果表明，衍生化颗粒诱导的细胞行为和形态改变与普通颗粒不同，这表明细胞反应可以通过特定设计的颗粒表面来引导。
