Berry Catherine C, Charles Stuart, Wells Stephen, Dalby Matthew J, Curtis Adam S G
Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, Scotland, UK.
Int J Pharm. 2004 Jan 9;269(1):211-25. doi: 10.1016/j.ijpharm.2003.09.042.
Magnetic nanoparticles have been used for bio-medical purposes including drug delivery, cell destruction and as MRI contrast agents for several years. A more recent biological application has focused on targeted drug delivery. To this end, a wide variety of iron oxide nanoparticles have been synthesised. This study involves the use of magnetic nanoparticles synthesised and derivatised with human transferrin, compared to identical underivatised particles. Human fibroblasts were used, representative of a tissue cell-type. The influence in vitro was determined using light and fluorescence microscopy, scanning and transmission electron microscopy, and 1718 gene microarray. The results indicate that the transferrin derivatised particles appear to localise to the cell membrane without instigating receptor-mediated endocytosis, and also induce up-regulation in the cells for many genes, particularly in the area of cytoskeleton and cell signalling. The microscopy results further support these findings.
磁性纳米颗粒用于生物医学目的已有数年，包括药物递送、细胞破坏以及作为磁共振成像（MRI）造影剂。最近的一项生物学应用聚焦于靶向药物递送。为此，人们已经合成了各种各样的氧化铁纳米颗粒。本研究涉及使用与人转铁蛋白合成并衍生化的磁性纳米颗粒，并与相同的未衍生化颗粒进行比较。使用人成纤维细胞，其代表一种组织细胞类型。通过光学和荧光显微镜、扫描和透射电子显微镜以及1718基因微阵列测定体外影响。结果表明，转铁蛋白衍生化的颗粒似乎定位于细胞膜，而不会引发受体介导的内吞作用，并且还会诱导细胞中许多基因的上调，特别是在细胞骨架和细胞信号传导方面。显微镜检查结果进一步支持了这些发现。
