Perales J, Amorim C Z, Rocha S L, Domont G B, Moussatché H
Departamento de Bioquímica, Universidad Centro Occidental, Barquisimeto, Venezuela.
Agents Actions. 1992 Nov;37(3-4):250-9. doi: 10.1007/BF02028117.
The pharmacological modulation of mice paw oedema produced by Bothrops jararaca venom (BJV) has been studied. Intraplantar injection of BJV (1-30 micrograms/paw) produced a dose- and time-related oedema, which was maximal 30 min after injection, reduced gradually thereafter and disappeared over 48 h. BJV heated at 100 degrees C for 5 or 15 min blocked local hemorrhage and caused partial inhibition of its oedematogenic activity. The BJV oedema was not inhibited by the anti-histamine meclizine, the inhibitor of histamine and serotonin, cyproheptadine, PAF-acether antagonist WEB 2170 or by the anti-leukotrienes C4/D4, LY 171883. Dexamethasone, aspirin, indomethacin, and the dual cyclooxygenase and lipoxygenase inhibitor BW 755C inhibited BJV-induced oedema indicating that arachidonic acid metabolism products via the cyclooxygenase pathway participate in its genesis and/or maintenance. The antibothropic fraction (ABF) (25-200 micrograms/paw) isolated from Didelphis marsupialis serum neutralized the oedema induced by the venom with and without heating, the hemorrhage induced by BJV and partially blocked the oedema induced by bradykinin and by cellulose sulphate. The oedema produced by histamine, serotonin, PAF-acether or leukotriene C4 was not inhibited.
对由巴西矛头蝮蛇毒（BJV）引起的小鼠爪部水肿的药理调节作用进行了研究。足底注射BJV（1 - 30微克/爪）可产生与剂量和时间相关的水肿，注射后30分钟水肿达到最大值，此后逐渐减轻，并在48小时内消失。在100℃加热5或15分钟的BJV可阻止局部出血，并部分抑制其致水肿活性。BJV引起的水肿不受抗组胺药美克洛嗪、组胺和5-羟色胺抑制剂赛庚啶、PAF-乙酰醚拮抗剂WEB 2170或抗白三烯C4/D4药物LY 171883的抑制。地塞米松、阿司匹林、吲哚美辛以及环氧化酶和脂氧化酶双重抑制剂BW 755C可抑制BJV诱导的水肿，这表明通过环氧化酶途径产生的花生四烯酸代谢产物参与了其发生和/或维持过程。从负鼠血清中分离出的抗矛头蝮蛇毒组分（ABF）（25 - 200微克/爪）可中和毒液加热或未加热时诱导的水肿、BJV诱导的出血，并部分阻断缓激肽和硫酸纤维素诱导的水肿。组胺、5-羟色胺、PAF-乙酰醚或白三烯C4引起的水肿未受抑制。
