Araújo S Dias, de Souza A, Nunes F P B, Gonçalves L R C
Laboratório de Fisiopatologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900, São Paulo, SP, Brazil.
Inflamm Res. 2007 Oct;56(10):409-13. doi: 10.1007/s00011-007-7054-x.
The present study investigates the supposed advantage of using an association of anti-inflammatory drugs and serum therapy to treat mouse paw edema induced by injection of Bothrops jararaca snake venom (BjV).
Edema was induced by injecting BjV (2 microg) into the footpad of male Swiss mice (20-25 g) and measured by plethysmography. Groups of mice were treated 15, 30 or 45 min after BjV injection with Bothrops antivenom or anti-inflammatory drugs (dexamethasone, indomethacin or zileuton), or with the association of antivenom and each one of these drugs.
Antivenom, dexamethasone and indomethacin were effective in reducing the paw edema when used up to 30 min after BjV injection. Zileuton had the same effect, but only if used up to 15 min after BjV injection. The association of antivenom and dexamethasone showed the greatest inhibitory effect when used up to 45 min after BjV injection. At this time, antivenom or anti-inflammatory drugs administered alone were ineffective.
Results suggest that dexamethasone combination with serum therapy can be beneficial for treatment of inflammatory edema caused by B. jararaca envenomation.
本研究调查使用抗炎药物与血清疗法联合治疗由注射巴西矛头蝮蛇毒（BjV）引起的小鼠爪肿胀的假定优势。
通过向雄性瑞士小鼠（20 - 25克）的足垫注射BjV（2微克）诱导肿胀，并用体积描记法测量。在注射BjV后15、30或45分钟，用巴西矛头蝮抗蛇毒血清或抗炎药物（地塞米松、吲哚美辛或齐留通），或用抗蛇毒血清与这些药物中的每一种联合治疗小鼠组。
抗蛇毒血清、地塞米松和吲哚美辛在BjV注射后30分钟内使用时可有效减轻爪肿胀。齐留通也有相同效果，但仅在BjV注射后15分钟内使用时有效。抗蛇毒血清与地塞米松联合在BjV注射后45分钟内使用时显示出最大的抑制作用。此时，单独给予抗蛇毒血清或抗炎药物无效。
结果表明地塞米松与血清疗法联合可能有益于治疗由巴西矛头蝮蛇咬伤引起的炎性肿胀。
