Lomonte B, Cerdas L, Gené J A, Gutiérrez J M
Toxicon. 1982;20(3):571-9. doi: 10.1016/0041-0101(82)90051-4.
The neutralizing capacity of the blood serum of the non-venomous snake Clelia clelia against the hemorrhagic, edematous and myonecrotic effects of Bothrops asper venom in white mice was tested using in vitro preincubation experiments. Untreated serum neutralized to a different extent all local effects evaluated, but showed toxicity to mice, evidenced by edema and myonecrosis. Serum heated at 56 degrees C for 30 min lost its toxic properties but also its neutralizing capacity against hemorrhagic and edematous effects of the venom. The myonecrotic effect was neutralized by large quantities of heated serum. The serum of C. clelia formed a single precipitation arc in immunoelectrophoretic slides against B. asper venom, corresponding to the slow anodic proteins of the serum.
利用体外预孵育实验，测试了无毒蛇Clelia clelia血清对白鼠体内矛头蝮蛇毒的出血、水肿和肌坏死效应的中和能力。未经处理的血清在不同程度上中和了所有评估的局部效应，但对小鼠显示出毒性，表现为水肿和肌坏死。在56℃加热30分钟的血清失去了毒性，但也失去了对蛇毒出血和水肿效应的中和能力。大量加热的血清可中和肌坏死效应。Clelia clelia血清在免疫电泳载玻片上与矛头蝮蛇毒形成单一沉淀弧，对应于血清中的慢阳极蛋白。
