基于5'核酸酶的实时荧光定量PCR检测方法的建立，用于定量检测致龋性口腔病原菌变形链球菌和远缘链球菌。
Development of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus.
作者信息
Yoshida Akihiro, Suzuki Nao, Nakano Yoshio, Kawada Miki, Oho Takahiko, Koga Toshihiko
机构信息
Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan.
出版信息
J Clin Microbiol. 2003 Sep;41(9):4438-41. doi: 10.1128/JCM.41.9.4438-4441.2003.
Abstract
A 5' nuclease TaqMan PCR assay was developed for the quantitative detection of the major cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. The absolute and relative numbers of bacteria were measured by this method. This assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation.
摘要
开发了一种5'核酸酶TaqMan PCR检测法，用于定量检测主要致龋菌变形链球菌和远缘链球菌。通过该方法测定细菌的绝对数量和相对数量。该检测法将有助于定量口腔标本中的这些微生物，并分析生物膜的形成。
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2
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FEMS Microbiol Lett. 2002 Nov 19;217(1):23-30. doi: 10.1111/j.1574-6968.2002.tb11451.x.
3
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4
Immunization against dental caries.预防龋齿的免疫接种。
Vaccine. 2002 May 15;20(16):2027-44. doi: 10.1016/s0264-410x(02)00047-6.
5
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Curr Issues Mol Biol. 2001 Apr;3(2):35-6.
6
Identification of Actinobacillus actinomycetemcomitans serotypes by multiplex PCR.通过多重聚合酶链反应鉴定伴放线放线杆菌血清型
J Clin Microbiol. 2001 May;39(5):2002-5. doi: 10.1128/JCM.39.5.2002-2005.2001.
7
Simple and rapid detection of Streptococcus mutans and Streptococcus sobrinus in human saliva by polymerase chain reaction.通过聚合酶链反应简单快速检测人唾液中的变形链球菌和远缘链球菌。
Oral Microbiol Immunol. 2000 Aug;15(4):258-62. doi: 10.1034/j.1399-302x.2000.150408.x.
8
Quantitative real-time PCR for Porphyromonas gingivalis and total bacteria.牙龈卟啉单胞菌和总细菌的定量实时聚合酶链反应
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9
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Methods Enzymol. 1999;310:109-16. doi: 10.1016/s0076-6879(99)10009-0.
10
Host-pathogen interactions in bacterial endocarditis: streptococcal virulence in the host.
Adv Dent Res. 1997 Apr;11(1):69-74. doi: 10.1177/08959374970110011901.
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