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基于5'核酸酶的实时荧光定量PCR检测方法的建立，用于定量检测致龋性口腔病原菌变形链球菌和远缘链球菌。

Development of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus.

作者信息

Yoshida Akihiro, Suzuki Nao, Nakano Yoshio, Kawada Miki, Oho Takahiko, Koga Toshihiko

机构信息

Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan.

出版信息

J Clin Microbiol. 2003 Sep;41(9):4438-41. doi: 10.1128/JCM.41.9.4438-4441.2003.

DOI:10.1128/JCM.41.9.4438-4441.2003
PMID:12958287
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC193796/
Abstract

A 5' nuclease TaqMan PCR assay was developed for the quantitative detection of the major cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. The absolute and relative numbers of bacteria were measured by this method. This assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation.

摘要

开发了一种5'核酸酶TaqMan PCR检测法，用于定量检测主要致龋菌变形链球菌和远缘链球菌。通过该方法测定细菌的绝对数量和相对数量。该检测法将有助于定量口腔标本中的这些微生物，并分析生物膜的形成。

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J Med Microbiol. 2002 May;51(5):443-447. doi: 10.1099/0022-1317-51-5-443.
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Immunization against dental caries.预防龋齿的免疫接种。
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