作为从慢性肝脓肿中分离出羊布鲁氏菌的工具的壳瓶培养法
Shell vial culture as a tool for isolation of Brucella melitensis in chronic hepatic abscess.
作者信息
Rovery Clarisse, Rolain Jean Marc, Raoult Didier, Brouqui Philippe
机构信息
Service de Maladies Infectieuses et de Médecine Tropicale, Hôpital Nord, AHPM, Marseille, France.
出版信息
J Clin Microbiol. 2003 Sep;41(9):4460-1. doi: 10.1128/JCM.41.9.4460-4461.2003.
Abstract
A 50-year-old man was admitted with a liver abscess and positive serology for Brucella spp. Liver pus and blood cultures remained sterile on conventional culture. Inoculation of liver pus onto eukaryotic cells by the centrifugation-shell vial technique yielded Brucella melitensis, identified by 16S rRNA gene amplification and sequencing.
摘要
一名50岁男性因肝脓肿和布鲁氏菌属血清学阳性入院。肝脓肿脓液和血培养在常规培养下均无菌生长。采用离心管小瓶技术将肝脓肿脓液接种于真核细胞，经16S rRNA基因扩增和测序鉴定为羊种布鲁氏菌。
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