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本文引用的文献

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Chronic localized brucellosis, with recurrent constitutional manifestations.慢性局限性布鲁氏菌病,伴有反复的全身症状。
Arch Intern Med. 1961 Jan;107:75-80. doi: 10.1001/archinte.1961.03620010079013.
2
Current understanding and management of chronic hepatosplenic suppurative brucellosis.慢性肝脾化脓性布鲁氏菌病的当前认识与管理
Clin Infect Dis. 2001 Apr 1;32(7):1024-33. doi: 10.1086/319608. Epub 2001 Mar 19.
3
16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates.对大量环境和临床来源无法鉴定的细菌分离株进行16S核糖体DNA序列分析。
J Clin Microbiol. 2000 Oct;38(10):3623-30. doi: 10.1128/JCM.38.10.3623-3630.2000.
4
Isolation in endothelial cell cultures of chlamydia trachomatis LGV (Serovar L2) from a lymph node of a patient with suspected cat scratch disease.从一名疑似猫抓病患者的淋巴结中,在内皮细胞培养物中分离出沙眼衣原体LGV(血清型L2)。
J Clin Microbiol. 2000 Jun;38(6):2062-4. doi: 10.1128/JCM.38.6.2062-2064.2000.
5
Isolation of mycobacteria from clinical samples using the centrifugation-shell vial technique.采用离心-壳管瓶技术从临床样本中分离分枝杆菌。
Eur J Clin Microbiol Infect Dis. 2000 Jan;19(1):69-70. doi: 10.1007/s100960050015.
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Cultivation of the bacillus of Whipple's disease.惠普尔病杆菌的培养
N Engl J Med. 2000 Mar 2;342(9):620-5. doi: 10.1056/NEJM200003023420903.
7
Isolation of Legionella pneumophila by centrifugation of shell vial cell cultures from multiple liver and lung abscesses.通过对来自多个肝肺脓肿的壳瓶细胞培养物进行离心分离嗜肺军团菌。
J Clin Microbiol. 1999 Mar;37(3):785-7. doi: 10.1128/JCM.37.3.785-787.1999.
8
Isolation of Francisella tularensis by centrifugation of shell vial cell culture from an inoculation eschar.通过对来自接种焦痂的壳瓶细胞培养物进行离心分离土拉弗朗西斯菌。
J Clin Microbiol. 1998 Sep;36(9):2782-3. doi: 10.1128/JCM.36.9.2782-2783.1998.
9
Diagnosis of Mediterranean spotted fever by cultivation of Rickettsia conorii from blood and skin samples using the centrifugation-shell vial technique and by detection of R. conorii in circulating endothelial cells: a 6-year follow-up.采用离心管瓶技术从血液和皮肤样本中培养康氏立克次体以及检测循环内皮细胞中的康氏立克次体来诊断地中海斑疹热:一项6年随访研究
J Clin Microbiol. 1996 Nov;34(11):2722-7. doi: 10.1128/jcm.34.11.2722-2727.1996.
10
Hepatosplenic abscesses due to Brucella melitensis: report of a case involving a child and review of the literature.布鲁氏菌病所致肝脾脓肿:1例儿童病例报告及文献复习
Clin Infect Dis. 1996 Mar;22(3):485-9. doi: 10.1093/clinids/22.3.485.

作为从慢性肝脓肿中分离出羊布鲁氏菌的工具的壳瓶培养法

Shell vial culture as a tool for isolation of Brucella melitensis in chronic hepatic abscess.

作者信息

Rovery Clarisse, Rolain Jean Marc, Raoult Didier, Brouqui Philippe

机构信息

Service de Maladies Infectieuses et de Médecine Tropicale, Hôpital Nord, AHPM, Marseille, France.

出版信息

J Clin Microbiol. 2003 Sep;41(9):4460-1. doi: 10.1128/JCM.41.9.4460-4461.2003.

DOI:10.1128/JCM.41.9.4460-4461.2003
PMID:12958294
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC193838/
Abstract

A 50-year-old man was admitted with a liver abscess and positive serology for Brucella spp. Liver pus and blood cultures remained sterile on conventional culture. Inoculation of liver pus onto eukaryotic cells by the centrifugation-shell vial technique yielded Brucella melitensis, identified by 16S rRNA gene amplification and sequencing.

摘要

一名50岁男性因肝脓肿和布鲁氏菌属血清学阳性入院。肝脓肿脓液和血培养在常规培养下均无菌生长。采用离心管小瓶技术将肝脓肿脓液接种于真核细胞,经16S rRNA基因扩增和测序鉴定为羊种布鲁氏菌。