Skaudickas Darijus, Kondrotas Anatolijus Juozas, Baltrusaitis Kestutis, Vaitiekaitis Gintautas
Department of Physiology, Kaunas University of Medicine, 3000 Kaunas, Lithuania.
Medicina (Kaunas). 2003;39(8):761-6.
This work has investigated the effect of echinacea extract on the weight of prostates in rats as well as on alterations of hystological structure and separate blood cells. This preparation was chosen for investigations due to its possible antiandrogenic effect and good immunostimulating features. Experiments were carried out with three-month old male Wistar rats, divided by six into three different groups. The first group was the control one. The rats of the second group were fed for 30 days with the usual food ration plus 50 mg/kg of echinacea extract. The third group was fed for 60 days in the same way as the second one. Clinical death of rats was caused by sodium phenobarbital, later a cervical dislocation was performed. After weighing the rats their prostates were removed and weighed. Patohystological investigations of the removed organs were carried out. Blood test for Shiling's analysis was taken. The weight of prostates in the first group of rats was 412.0+/-14.93 mg, in the second group - 403.0+/-13.33 mg, and in the third group it was 388.0+/-14.66 mg. Having calculated the proportion between prostates of rats and their body weight it was estimated that in the first group it made 0.125+/-0.009%, in the second group - 0.105+/-0.005%, and in the third group - 0.091+/-0.007%. The percentage of lymphocytes in the first group was 72+/-1.41; in the second group - 73+/-0.81; in the third group - 79+/-1,86. The percentage of segmented neutrophyle in the first group was 23+/-3.31; in the second group - 23+/-2.25; in the third group - 18+/-2.33. Having conducted analysis of the experimental results, a significantly important decrease of prostate weight of investigated rats, an increase in the number of lymphocyte as well as the alterations of hystological structures after using echinacea extract for eight weeks were observed.
本研究调查了紫锥菊提取物对大鼠前列腺重量以及组织学结构和单个血细胞变化的影响。选择该制剂进行研究是因其可能具有抗雄激素作用和良好的免疫刺激特性。实验使用三个月大的雄性Wistar大鼠，将其六只分为三组。第一组为对照组。第二组大鼠用常规食物日粮加50毫克/千克紫锥菊提取物喂养30天。第三组以与第二组相同的方式喂养60天。用苯巴比妥钠致大鼠临床死亡，随后进行颈椎脱臼。称重大鼠后，取出并称重其前列腺。对取出的器官进行病理组织学研究。进行希林氏分析的血液检测。第一组大鼠前列腺重量为412.0±14.93毫克，第二组为403.0±13.33毫克，第三组为388.0±14.66毫克。计算大鼠前列腺与体重的比例后估计，第一组为0.125±0.009%，第二组为0.105±0.005%，第三组为0.091±0.007%。第一组淋巴细胞百分比为72±1.41；第二组为73±0.81；第三组为79±1.86。第一组分叶中性粒细胞百分比为23±3.31；第二组为23±2.25；第三组为18±2.33。对实验结果进行分析后，观察到使用紫锥菊提取物八周后，被研究大鼠的前列腺重量显著下降、淋巴细胞数量增加以及组织学结构改变。
