南瓜籽油和植物甾醇-F可抑制睾酮/哌唑嗪诱导的大鼠前列腺生长。

Pumpkin seed oil and phytosterol-F can block testosterone/prazosin-induced prostate growth in rats.

作者信息

Tsai Yuh-Shyan, Tong Yat-Ching, Cheng Juei-Tang, Lee Chung-Ho, Yang Fu-Shan, Lee Hua-Yang

机构信息

Institute of Clinical Medicine, Department of Urology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.

出版信息

Urol Int. 2006;77(3):269-74. doi: 10.1159/000094821.

DOI:10.1159/000094821

PMID:17033217

Abstract

INTRODUCTION

This study was undertaken to investigate the effects of pumpkin seed oil alone or combined with Phytosterol-F on testosterone/prazosin-induced (T-P) prostate growth in rats.

MATERIALS AND METHODS

Forty adult Wistar rats were divided into five groups, including: one control group, rats treated with vehicle only, one group treated with T-P, and two groups of T-P-treated rats, one receiving orally pumpkin seed oil alone and one group receiving orally pumpkin seed oil combined with Phytosterol-F. Two weeks later, the prostatic weight-to-body weight ratio was determined after sacrifice. The total protein concentration was measured by using a protein assay. Some ventral prostatic tissues were histologically examined after hematoxylin-eosin staining.

RESULTS

Histological sections of the ventral prostate showed that the architecture of the prostate glands became hyperplastic in the T-P rats, but not in the control or vehicle-treated animals. As compared with the control or vehicle group, T-P rats had a significantly higher prostatic weight-to-body weight ratio for the ventral prostate (p=0.05 and p=0.007, respectively), but not for the dorsolateral prostate (p=0.53 and p=0.73, respectively). The T-P rats had significantly higher protein levels within both lobes (ventral lobe, p=0.02 and p<0.0001, respectively; dorsolateral lobe, p=0.06 and p=0.005, respectively). As compared with the T-P-alone rats, the TP rats treated with pumpkin seed oil alone or pumpkin seed oil combined with Phytosterol-F had a significantly lower weight ratio for the ventral prostate (p=0.01 and p=0.004, respectively) and significantly lower protein levels within both lobes (p=0.03 and p=0.003, respectively; p=0.007 and p=0.002, respectively). In addition, Phytosterol-F had some additive effect on the total protein synthesis within the ventral prostate (p=0.02).

CONCLUSION

Pumpkin seed oil alone or combined with Phytosterol-F can block the T-P-induced increases in prostatic weight-to-body weight ratio and protein synthesis.

摘要

引言

本研究旨在探讨南瓜籽油单独使用或与植物甾醇 - F联合使用对睾酮/哌唑嗪诱导（T - P）的大鼠前列腺生长的影响。

材料与方法

将40只成年Wistar大鼠分为五组，包括：一个对照组，仅用赋形剂处理的大鼠，一组用T - P处理的大鼠，以及两组用T - P处理的大鼠，一组口服单独的南瓜籽油，一组口服南瓜籽油与植物甾醇 - F的组合。两周后，处死大鼠后测定前列腺重量与体重之比。使用蛋白质测定法测量总蛋白浓度。苏木精 - 伊红染色后对一些腹侧前列腺组织进行组织学检查。

结果

腹侧前列腺的组织学切片显示，T - P大鼠的前列腺腺泡结构增生，但对照组或用赋形剂处理的动物中未出现这种情况。与对照组或赋形剂组相比，T - P大鼠腹侧前列腺的前列腺重量与体重之比显著更高（分别为p = 0.05和p = 0.007），但背外侧前列腺的该比值无显著差异（分别为p = 0.53和p = 0.73）。T - P大鼠两个叶内的蛋白水平均显著更高（腹侧叶，分别为p = 0.02和p < 0.0001；背外侧叶，分别为p = 0.06和p = 0.005）。与仅用T - P处理的大鼠相比，单独口服南瓜籽油或口服南瓜籽油与植物甾醇 - F组合的T - P大鼠腹侧前列腺的重量比显著更低（分别为p = 0.01和p = 0.004），两个叶内的蛋白水平也显著更低（分别为p = 0.03和p = 0.003；分别为p = 0.007和p = 0.002）。此外，植物甾醇 - F对腹侧前列腺内的总蛋白合成有一定的相加作用（p = 0.02）。