Kaneuchi Masanori, Sasaki Masahiro, Tanaka Yuichiro, Yamamoto Ritsu, Sakuragi Noriaki, Dahiya Rajvir
Department of Urology, San Francisco and Veterans Affairs Medical Center, University of California, San Francisco, CA 94121, USA.
Int J Oncol. 2003 Oct;23(4):1167-72.
Resveratrol and other polyphenols have anti-carcinogenic and anti-tumorigenic activities in various carcinomas. However, such studies are limited in endometrial cancer. We hypothesize that resveratrol suppresses cancer growth through modulation of cell cycle and cell growth regulatory genes. To test this hypothesis, we treated endometrial cancer cells (Ishikawa cell line) with resveratrol (1, 10, 50 and 100 micro M) for 1, 3, 5 and 7 days, and analyzed for growth signal genes (EGF and VEGF), cell cycle regulatory genes (p53 and p21), and apoptosis-related genes (bcl-2 and bax). Results of these experiments demonstrate that after resveratrol treatment, the growth of Ishikawa cells was inhibited in a dose dependent manner. The gene and protein expression data suggest that resveratrol treatment significantly decreased EGF, whereas VEGF was up-regulated in Ishiwaka cell lines. Interestingly, protein expressions of p21 and Bax were decreased, even though their mRNA expressions did not show significant changes. The present study suggests that resveratrol can suppress proliferation of Ishikawa cells through down-regulation of EGF.
白藜芦醇和其他多酚类物质在多种癌症中具有抗癌和抗肿瘤活性。然而，此类研究在子宫内膜癌方面较为有限。我们推测白藜芦醇通过调节细胞周期和细胞生长调控基因来抑制癌症生长。为验证这一推测，我们用白藜芦醇（1、10、50和100微摩尔）处理子宫内膜癌细胞（石川细胞系）1、3、5和7天，并分析生长信号基因（表皮生长因子和血管内皮生长因子）、细胞周期调控基因（p53和p21）以及凋亡相关基因（bcl-2和bax）。这些实验结果表明，白藜芦醇处理后，石川细胞的生长呈剂量依赖性抑制。基因和蛋白质表达数据表明，白藜芦醇处理显著降低了表皮生长因子，而血管内皮生长因子在石川细胞系中上调。有趣的是，尽管p21和Bax的mRNA表达未显示出显著变化，但其蛋白质表达却降低了。本研究表明，白藜芦醇可通过下调表皮生长因子来抑制石川细胞的增殖。
