Fernandes Everton Kort Kamp, da Costa Gisela Lara, de Souza Edson Jesus, de Moraes Aurea Maria Lage, Bittencourt Vania Rita Elias Pinheiro
Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, Brazil.
J Basic Microbiol. 2003;43(5):393-8. doi: 10.1002/jobm.200310263.
The purpose of this work was to evaluate the in vitro virulence of three isolates of Beauveria bassiana to eggs and larvae of the tick Boophilus microplus. The fungus tested were isolated from engorged females of B. microplus collected on the field, and identified as Bb28, Bb29 and Bb30. These isolates were evaluated by immersion of eggs and larvae in suspensions with different conidial concentrations: 10(8), 10(7), 10(6) and 10(5) conidia/ml and compared to the control groups. In the treated eggs, there was a percentage much smaller of hatching than that observed in the controls. The egg hatch was inversely proportional to the conidial concentration. In larval bioassays, all isolates resulted in a higher mortality of larvae compared to the control according to the conidial concentrations/ml, 10 days after treatment inoculation.
本研究旨在评估球孢白僵菌的三株分离株对微小牛蜱卵和幼虫的体外毒力。所测试的真菌是从野外采集的饱血微小牛蜱雌虫中分离得到的，分别鉴定为Bb28、Bb29和Bb30。通过将卵和幼虫浸泡在不同分生孢子浓度（10⁸、10⁷、10⁶和10⁵个分生孢子/毫升）的悬浮液中对这些分离株进行评估，并与对照组进行比较。在处理过的卵中，孵化的百分比远低于对照组。卵孵化率与分生孢子浓度成反比。在幼虫生物测定中，处理接种10天后，根据每毫升分生孢子浓度，与对照组相比，所有分离株均导致幼虫死亡率更高。
