Dykes P J, Heggie R, Hill S A
Department of Dermatology, University of Wales College of Medicine, Cardiff, UK.
J Wound Care. 2001 Feb;10(2):7-10. doi: 10.12968/jowc.2001.10.2.26054.
Two human models were developed to quantify the stratum corneum removed by different adhesive dressings and to measure the peel force of dressing removal and relate this to stratum corneum removal. The first was an open study designed to compare the effects of applying Mepiform Safetac, Tielle and Duoderm Extra Thin to the skin of 12 normal volunteers aged 19-53 years. Treatments were applied once (one 24-hour application) or three times (three x 24-hour applications) to forearm skin which had been prestained with methylene blue. After dressing removal the dye left on the skin was sampled using the skin surface biopsy method and measured spectrophotometrically. The results show that, after one and three applications, the Mepiform Safetac sites had a higher level of dye than those on which the other dressings had been applied (p < 0.05, after three applications). Based on the assumption that the more dye is left on the skin, the less damage is caused, this suggests that Mepiform Safetac is less damaging to the skin surface than the other products tested. In the second study the peel force needed to remove adhesive dressings from prestained skin was measured and related to the amount of stratum corneum removed. Mepilex Border Safetac, Duoderm Extra Thin, Allevyn Adhesive, Biatain Adhesive and Tielle Hydropolymer Dressing were compared in 20 normal volunteers aged 23-64 years. Three consecutive 24-hour applications of each product were made, with measurements of peel force at 24, 48 and 72 hours. The amount of dye remaining on the skin at 72 hours was assessed by the surface biopsy method. Statistically significant differences between products were observed in terms of both peak force and steady state force of removal. Differences in the level of damage to the superficial stratum corneum were also detected. However, low levels of peel force were not always associated with low damage and, therefore, other factors must contribute to stratum corneum removal in this model.
开发了两种人体模型，以量化不同粘性敷料去除的角质层，并测量去除敷料的剥离力，并将其与角质层去除情况相关联。第一个是开放性研究，旨在比较将美皮康Safetac、蒂爱尔和多爱肤超薄敷料应用于12名年龄在19至53岁的正常志愿者皮肤上的效果。将处理剂应用一次（一次24小时应用）或三次（三次×24小时应用）于预先用亚甲蓝染色的前臂皮肤。去除敷料后，使用皮肤表面活检方法对留在皮肤上的染料进行采样，并通过分光光度法进行测量。结果表明，在一次和三次应用后，美皮康Safetac部位的染料水平高于应用其他敷料的部位（三次应用后，p<0.05）。基于留在皮肤上的染料越多，造成的损伤越小这一假设，这表明美皮康Safetac对皮肤表面的损伤小于其他测试产品。在第二项研究中，测量了从预先染色的皮肤上去除粘性敷料所需的剥离力，并将其与去除的角质层量相关联。在20名年龄在23至64岁的正常志愿者中比较了美皮贴Border Safetac、多爱肤超薄敷料、爱立肤粘性敷料、比亚芬粘性敷料和蒂爱尔水凝胶敷料。每种产品连续进行三次24小时应用，并在24、48和72小时测量剥离力。通过表面活检方法评估72小时时留在皮肤上的染料量。在去除的峰值力和稳态力方面观察到产品之间存在统计学上的显著差异。还检测到对浅表角质层的损伤程度存在差异。然而，低水平的剥离力并不总是与低损伤相关，因此，在该模型中，其他因素必定对角质层去除有影响。
