Bezrukova I P, Malkov M A
Antibiot Khimioter. 1992 Oct;37(10):24-6.
The impact of the vegetative inoculum parameters on specificity of the secondary synthesis in the cultures producing novobiocin and mycoheptin was studied. During the study the fermentation conditions were varied by using the vegetative inoculum differing in the respiration rate after its transfer to the fermentation medium. To show the decisive role of the inoculum parameters in regulation of the specificity of the secondary synthesis, the dynamics of accumulation of certain metabolites forming from glucose along with the main antibiotic and the activity of the key enzymes of the carbohydrate metabolism during the culture growth in the fermentation media were studied. It was found that the specificity of the secondary synthesis with respect to certain metabolites was defined by the intensity of carbohydrate metabolism, i. e. the ratio of the activity of enzymes of glycolysis and the pentosephosphate pathway. In this regard, the inoculum with the maximum respiration rate in an amount of 10 to 20 per cent promoted the highest productivity of the mycelium by the synthesis of novobiocin and mycoheptin while the rate of accumulation of fatty acids, carbohydrates and phenol compounds (for Streptomyces spheroides) and mycopentene (for Streptoverticillium mycoheptinicum) decreased.
研究了营养接种物参数对新霉素和霉肝褐菌素产生菌培养物中二次合成特异性的影响。在研究过程中，通过使用转移至发酵培养基后呼吸速率不同的营养接种物来改变发酵条件。为了表明接种物参数在二次合成特异性调节中的决定性作用，研究了在发酵培养基中培养期间，由葡萄糖与主要抗生素一起形成的某些代谢物的积累动态以及碳水化合物代谢关键酶的活性。发现二次合成对某些代谢物的特异性由碳水化合物代谢强度决定，即糖酵解酶和磷酸戊糖途径酶的活性之比。在这方面，呼吸速率最高的接种物用量为10%至20%时，通过新霉素和霉肝褐菌素的合成促进了菌丝体的最高生产力，而脂肪酸、碳水化合物和酚类化合物（对于球形链霉菌）以及霉戊烯（对于产霉肝褐菌素链孢囊菌）的积累速率降低。
