Vinnikov A I, Babenko Iu S
Antibiot Khimioter. 1989 Jan;34(1):34-8.
Intensity of glycolysis and the pentose phosphate cycle in staphylococci sensitive and resistant to novobiocin was studied. The resistant variants did not practically store lactate and the activity of glycolytic enzymes i.e. hexokinase and aldolase was lowered by 15-20 and 53-59 per cent, respectively. Monoiodoacetate, a glycolysis inhibitor suppressed the glucose oxidation rate by 53.3-66.9 per cent in the sensitive variants and by 16-21.8 per cent in the resistant variants. At the same time it was characteristic of the resistant variants to increase the activity of the pentose phosphate cycle enzymes; glucose-6-phosphate dehydrogenase by 25-38.1 per cent transketolase by 21.5-27.3 per cent and transaldolase by 30-57.1 per cent. No differences in the transhydrogenase reaction kinetics of both the novobiocin sensitive and the novobiocin resistant variants were observed.
研究了对新生霉素敏感和耐药的葡萄球菌中糖酵解和磷酸戊糖循环的强度。耐药变体几乎不储存乳酸，糖酵解酶即己糖激酶和醛缩酶的活性分别降低了15%-20%和53%-59%。糖酵解抑制剂碘乙酸钠使敏感变体的葡萄糖氧化率降低了53.3%-66.9%，使耐药变体的葡萄糖氧化率降低了16%-21.8%。同时，耐药变体的特征是磷酸戊糖循环酶的活性增加；6-磷酸葡萄糖脱氢酶增加了25%-38.1%，转酮醇酶增加了21.5%-27.3%，转醛醇酶增加了30%-57.1%。未观察到新生霉素敏感和耐药变体的转氢酶反应动力学有差异。
