Rieck Bernd, Schlaak Sabine
Chirurgische Klinik III, Städtisches Krankenhaus Hildesheim GmbH, 31134 Hildesheim, Weinberg 1, Germany.
Ann Plast Surg. 2003 Sep;51(3):294-300. doi: 10.1097/01.SAP.0000063758.16488.A9.
Recent research suggests that transplantation of adipocyte precursors may solve some of the problems encountered with autologous adipocyte transplantation. Numerous earlier and recent in vitro studies have proved that preadipocytes, under certain circumstances, will differentiate into adipocytes and will build up a vacuole. The in vivo control of the success to implant a single-cell suspension of viable preadipocytes and to breed them to mature fat cells has, until now, been lacking. In a Lewis rat model, preadipocytes were harvested by digestion of fat tissue with collagenase type II, separated by repeated centrifugation, incubated with PKH26 (a nontoxic fluorescent surface marker), and reimplanted into the donor animal. In a follow-up of 6 months, the gradual differentiation of the fluorescent graft cells toward adipocytes could be observed using the confocal microscope. The observed changes in the graft cells were in exact accordance with the authors' expectations, which were based on the in vitro studies in literature. This study encourages further research of manipulated preadipocytes, feeding patterns, and so forth.
近期研究表明，脂肪细胞前体细胞的移植或许能解决自体脂肪细胞移植中遇到的一些问题。众多早期及近期的体外研究已证实，前脂肪细胞在某些情况下会分化为脂肪细胞并形成液泡。然而，迄今为止，在体内控制植入存活前脂肪细胞单细胞悬液并使其发育为成熟脂肪细胞的成功率方面仍存在不足。在一个Lewis大鼠模型中，通过用II型胶原酶消化脂肪组织来获取前脂肪细胞，经反复离心分离，用PKH26（一种无毒荧光表面标志物）孵育，然后重新植入供体动物体内。在为期6个月的随访中，使用共聚焦显微镜可观察到荧光移植细胞逐渐向脂肪细胞分化。移植细胞中观察到的变化与作者基于文献中的体外研究得出的预期完全相符。这项研究鼓励对经过处理的前脂肪细胞、喂养模式等进行进一步研究。
