Woods R D, Kunkle R A, Ridpath J E, Bolin S R
Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA.
J Vet Diagn Invest. 1999 Sep;11(5):400-7. doi: 10.1177/104063879901100503.
A bovine viral diarrhea virus (BVDV-C) was isolated from swine tissue culture cells used to attenuate the transmissible gastroenteritis virus (TGEV) after 68 passes. Piglets given a pure culture of BVDV-C developed clinical signs similar to those of a mild TGEV infection and recovered by 10 days postexposure. Villous blunting and fusion was observed in the small intestine, and a lymphocyte depletion was observed in Peyer's patches in the ileum. Piglets given a combination of BVDV-C and attenuated TGEV developed clinical signs similar to those of a virulent TGEV infection and were euthanized. The combined infection induced a generalized lymphocyte depletion throughout the lymphatic system and villous atrophy in the intestinal tract. Piglets exposed to a another type I strain of BVDV (NY-1) either alone or in combination with the attenuated TGEV had mild clinical signs similar to those of a TGEV infection. Moderate villous atrophy in the ileum and a lymphocyte depletion in the mesenteric lymph node were observed in these piglets postmortem. The data indicate a potential problem for diagnostic laboratories in relation to a diagnosis of virulent TGEV infections and in the field for young piglets exposed to a BVDV-contaminated TGEV vaccine.
一种牛病毒性腹泻病毒（BVDV-C）在68次传代后从用于减毒传染性胃肠炎病毒（TGEV）的猪组织培养细胞中分离出来。接种BVDV-C纯培养物的仔猪出现了与轻度TGEV感染相似的临床症状，并在接触后10天恢复。在小肠中观察到绒毛变钝和融合，在回肠的派伊尔氏淋巴集结中观察到淋巴细胞减少。接种BVDV-C和减毒TGEV组合的仔猪出现了与强毒TGEV感染相似的临床症状，并被实施安乐死。联合感染导致整个淋巴系统普遍淋巴细胞减少以及肠道绒毛萎缩。单独或与减毒TGEV联合接触另一种I型BVDV毒株（NY-1）的仔猪出现了与TGEV感染相似的轻度临床症状。这些仔猪死后在回肠中观察到中度绒毛萎缩，在肠系膜淋巴结中观察到淋巴细胞减少。数据表明，诊断实验室在诊断强毒TGEV感染方面以及在野外对于接触受BVDV污染的TGEV疫苗的仔猪而言存在潜在问题。
