Gómez-Romero Ninnet, Velazquez-Salinas Lauro, Ridpath Julia F, Verdugo-Rodríguez Antonio, Basurto-Alcántara Francisco Javier
Vaccinology Laboratory, Department of Microbiology and Immunology, Universidad Nacional Autonoma de México, 04510, Mexico City, Mexico.
Department of Anatomy and Physiology, Kansas State University, Manhattan, KS, 66506, USA.
Arch Virol. 2021 Jul;166(7):1999-2003. doi: 10.1007/s00705-021-05089-9. Epub 2021 May 10.
In this communication, we report the presence of RNA of bovine viral diarrhea virus (BVDV) as a contaminant of different biological products used in Mexico for veterinary vaccine production. For this purpose, six batches of monovalent vaccines, eight cell line batches used for vaccine production, and 10 fetal bovine serum lots (FBS) commercially available in Mexico from different suppliers were tested by reverse transcription polymerase chain reaction (RT-PCR). Viral RNA was detected in 62.5% of the samples analyzed. Phylogenetic analysis revealed the presence of the subgenotypes BVDV-1a, 1b, and BVDV-2a in the tested samples. Collectively, these findings indicate that contamination by BVDV RNA occurs in commercial vaccines and reagents used in research and production of biological products. The ramifications of this contamination are discussed.
在本通讯中，我们报告了牛病毒性腹泻病毒（BVDV）的RNA作为墨西哥用于兽用疫苗生产的不同生物制品的污染物而存在。为此，通过逆转录聚合酶链反应（RT-PCR）对墨西哥市场上来自不同供应商的6批单价疫苗、8批用于疫苗生产的细胞系批次以及10批胎牛血清（FBS）进行了检测。在所分析的样本中，62.5%检测到了病毒RNA。系统发育分析显示，在测试样本中存在BVDV-1a、1b和BVDV-2a亚基因型。总体而言，这些发现表明，BVDV RNA污染发生在生物制品研究和生产中使用的商业疫苗和试剂中。本文讨论了这种污染的影响。
