Tang Deh-Wei, Lin Shu-Chun, Chang Kuo-Wei, Chi Chin-Wen, Chang Che-Shoa, Liu Tsung-Yun
Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan.
J Oral Pathol Med. 2003 Oct;32(9):522-9. doi: 10.1034/j.1600-0714.2003.00182.x.
Elevated expression of cyclooxygenase (COX)-2 has been demonstrated in several human cancers. Whether COX-2 is up-regulated in areca quid (AQ) related oral squamous cell carcinoma (OSCC) is unknown and the potential of AQ ingredients to induce COX-2 expression has not been studied.
COX-2 expression was analyzed by immunohistochemistry and RT-PCR in oral tissues. The COX-2 mRNA and protein induction potential of AQ ingredients were analyzed by real-time RT-PCR and Western blotting in normal human oral keratinocyte (NHOK).
COX-2 protein expression was significantly higher (P < 0.01) in OSCC (n = 27) as compared to their adjacent non-cancerous matched tissue (NCMT). COX-2 protein was nearly undetectable in control normal oral mucosa. The level of COX-2 mRNA was markedly elevated in 63% (12/19) of OSCC compared to NCMT. Hydroxychavicol induced COX-2 mRNA and protein expression in NHOK.
COX-2 protein as well as mRNA expression were significantly enhanced in OSCC as compared to NCMT. Hydroxychavicol, a unique ingredient in AQ, induced COX-2 expression in NHOK, which highlighted early involvement of COX-2 in AQ-associated oral oncogenesis.
环氧化酶（COX）-2在多种人类癌症中呈现高表达。COX-2在槟榔相关口腔鳞状细胞癌（OSCC）中是否上调尚不清楚，且槟榔成分诱导COX-2表达的潜力尚未得到研究。
通过免疫组织化学和逆转录聚合酶链反应（RT-PCR）分析口腔组织中COX-2的表达。采用实时RT-PCR和蛋白质免疫印迹法分析槟榔成分对正常人口腔角质形成细胞（NHOK）中COX-2 mRNA和蛋白质的诱导潜力。
与配对的癌旁非癌组织（NCMT）相比，OSCC（n = 27）中COX-2蛋白表达显著更高（P < 0.01）。在对照正常口腔黏膜中几乎检测不到COX-2蛋白。与NCMT相比，63%（12/19）的OSCC中COX-2 mRNA水平显著升高。羟基查耳酮可诱导NHOK中COX-2 mRNA和蛋白表达。
与NCMT相比，OSCC中COX-2蛋白及mRNA表达均显著增强。槟榔中的独特成分羟基查耳酮可诱导NHOK中COX-2表达，这突出了COX-2在槟榔相关口腔肿瘤发生早期的作用。
