Maathuis Frans J M, Filatov Victor, Herzyk Pawel, Krijger Gerard C, Axelsen Kristian B, Chen Sixue, Green Brian J, Li Yi, Madagan Kathryn L, Sánchez-Fernández Rocío, Forde Brian G, Palmgren Michael G, Rea Philip A, Williams Lorraine E, Sanders Dale, Amtmann Anna
Department of Biology, University of York, PO Box 373, York YO10 5YW, UK.
Plant J. 2003 Sep;35(6):675-92. doi: 10.1046/j.1365-313x.2003.01839.x.
Plant nutrition critically depends on the activity of membrane transporters that translocate minerals from the soil into the plant and are responsible for their intra- and intercellular distribution. Most plant membrane transporters are encoded by multigene families whose members often exhibit overlapping expression patterns and a high degree of sequence homology. Furthermore, many inorganic nutrients are transported by more than one transporter family. These considerations, coupled with a large number of so-far non-annotated putative transporter genes, hamper our progress in understanding how the activity of specific transporters is integrated into a response to fluctuating conditions. We designed an oligonucleotide microarray representing 1096 Arabidopsis transporter genes and analysed the root transporter transcriptome over a 96-h period with respect to 80 mM NaCl, K+ starvation and Ca2+ starvation. Our data show that cation stress led to changes in transcript level of many genes across most transporter gene families. Analysis of transcriptionally modulated genes across all functional groups of transporters revealed families such as V-type ATPases and aquaporins that responded to all treatments, and families - which included putative non-selective cation channels for the NaCl treatment and metal transporters for Ca2+ starvation conditions - that responded to specific ionic environments. Several gene families including primary pumps, antiporters and aquaporins were analysed in detail with respect to the mRNA levels of different isoforms during ion stress. Cluster analysis allowed identification of distinct expression profiles, and several novel putative regulatory motifs were discovered within sets of co-expressed genes.
植物营养严重依赖于膜转运蛋白的活性，这些转运蛋白将矿物质从土壤转运到植物中，并负责其在细胞内和细胞间的分布。大多数植物膜转运蛋白由多基因家族编码，其成员通常表现出重叠的表达模式和高度的序列同源性。此外，许多无机养分由不止一个转运蛋白家族运输。这些因素，再加上大量迄今未注释的假定转运蛋白基因，阻碍了我们在理解特定转运蛋白的活性如何整合到对波动条件的响应方面取得进展。我们设计了一个代表1096个拟南芥转运蛋白基因的寡核苷酸微阵列，并在96小时内分析了80 mM NaCl、K+饥饿和Ca2+饥饿条件下根系转运蛋白转录组。我们的数据表明，阳离子胁迫导致大多数转运蛋白基因家族中许多基因的转录水平发生变化。对所有转运蛋白功能组中转录调控基因的分析揭示了对所有处理都有响应的家族，如V型ATP酶和水通道蛋白，以及对特定离子环境有响应的家族，包括NaCl处理的假定非选择性阳离子通道和Ca2+饥饿条件下的金属转运蛋白。对包括初级泵、反向转运蛋白和水通道蛋白在内的几个基因家族在离子胁迫期间不同异构体的mRNA水平进行了详细分析。聚类分析允许识别不同的表达谱，并且在共表达基因集中发现了几个新的假定调控基序。
