Kałowski M, Dybicki J, Mierzejewski W, Szelezyński K, Kedzia A, Kwapisz E, Jassem E, Lukiański M, Smiejkowski Z
Zakład Mikrobiologii Jamy Ustnej I.S. A.M., Gdańsku.
Med Dosw Mikrobiol. 1992;44(1-2):55-60.
For evaluation of usefulness of natural fluorescence of clinical materials in UV radiation as rapid diagnostic method of infections with anaerobes, 405 samples of pus, bloody-purulent fluids, blood, wound secretions, dressings and other materials were investigated. Occurrence of red-brick UV fluorescence of clinical materials was compared with results of culture aimed at isolation of non-sporeforming anaerobes from "B. melaninogenicus group (P. melaninogenica, P. intermedia and P. saccharolytics). Significant correlation red-brick fluorescence of clinical materials resulting from UV irradiation with presence in these materials of anaerobes such as P. melaninogenica, P. intermedia and P. asaccharolytics was detected. Investigation of clinical materials with application of fluorescence in UV radiation lasts only 1-2 minutes and together with preparation and microscopical inspection which is Gram-stained--only 15-20 min. Positive results of this test may constitute a basis for rapid, preliminary identification of the etiologic factor and for direction of chemotherapy in the early period of infection.
为评估临床材料在紫外线辐射下的自然荧光作为厌氧菌感染快速诊断方法的实用性，对405份脓液、血性脓性液体、血液、伤口分泌物、敷料及其他材料样本进行了研究。将临床材料红砖色紫外线荧光的出现情况与旨在从“产黑色素拟杆菌群（产黑色素普雷沃菌、中间普雷沃菌和解糖普雷沃菌）”中分离非芽孢厌氧菌的培养结果进行了比较。检测到临床材料经紫外线照射产生的红砖色荧光与这些材料中存在产黑色素普雷沃菌、中间普雷沃菌和非解糖普雷沃菌等厌氧菌之间存在显著相关性。应用紫外线辐射下的荧光对临床材料进行检测仅需1至2分钟，加上标本制备和革兰氏染色显微镜检查，总共仅需15至20分钟。该检测的阳性结果可为感染早期病因的快速初步鉴定及化疗方向提供依据。
