Tanaka J I, Takano N, Unozawa H, Shigematsu S, Kishino Y, Yonezu H, Matsuzaki H, Shigematsu T, Takahashi S
Tokyo Dental College.
Bull Tokyo Dent Coll. 1990 May;31(2):155-62.
The relationship between volatile fatty acids (VFAs) in pus and infecting bacterial species was examined in order to establish a rapid identification system for anaerobic microorganisms in purulent inflammation in the oro-maxillary region. VFAs were detected by the direct injection of pus into gas-liquid chromatography (GLC). Bacterial examination was carried out by anaerobic culture using blood agar plates. The bacterial identification was carried out mainly according to the VPI manual. Analysis of the direct VFA patterns of each sample resulted in 5 groups. The following bacterial species were the main isolates in each group: Streptococcus intermedius in Group A, Peptostreptococcus micros in Group B, Fusobacterium nucleatum in Group C, Bacteroides gingivalis in Group D, and Peptostreptococcus anaerobius in Group E. The profile of VFAs produced in the PYG culture medium of the above isolated bacteria was compared with the direct VFA patterns. Agreement ratios between direct and PYG VFA patterns were as follows: Group A, 47.1%; Groups B and C, 45.0%; Group D, 87.5%; and Group E, 62.9%. The acetic acid concentration was more than 14 x 10(-4) meq/ml in Group B, the butyric acid concentration was more than 7 x 10(-4) meq/ml in Group C, and the iso-caproic acid concentration was more than 14 x 10(-4) meq/ml in Group E. In these cases, it was found that the agreement ratios between the direct and PYG FVA pattern were high. In Group D, irrespective of the concentration of iso-valeric acid detected, the agreement ratio was very high. The antibiotic susceptibility of the isolates was studied. Efficiency rates of ABPC, PIPC, CCL, CEZ, CMZ, SBT/CPZ, JM, CLDM, MINO and GM were relatively low and resistant rates were high for the gram-negative rods.
为建立口腔颌面部化脓性炎症中厌氧微生物的快速鉴定系统，研究了脓液中挥发性脂肪酸（VFA）与感染细菌种类之间的关系。通过将脓液直接注入气液色谱（GLC）来检测VFA。使用血琼脂平板通过厌氧培养进行细菌检查。细菌鉴定主要依据VPI手册进行。对每个样本的直接VFA模式分析产生了5组。每组中的主要分离菌株如下：A组为中间链球菌，B组为微小消化链球菌，C组为具核梭杆菌，D组为牙龈拟杆菌，E组为厌氧消化链球菌。将上述分离细菌在PYG培养基中产生的VFA谱与直接VFA模式进行比较。直接和PYG VFA模式之间的一致率如下：A组为47.1%；B组和C组为45.0%；D组为87.5%；E组为62.9%。B组中乙酸浓度超过14×10⁻⁴ meq/ml，C组中丁酸浓度超过7×10⁻⁴ meq/ml，E组中异己酸浓度超过14×10⁻⁴ meq/ml。在这些情况下，发现直接和PYG FVA模式之间的一致率很高。在D组中，无论检测到的异戊酸浓度如何，一致率都非常高。研究了分离菌株的抗生素敏感性。对于革兰氏阴性杆菌，氨苄青霉素（ABPC）、哌拉西林（PIPC）、氯碳头孢（CCL）、头孢唑肟（CEZ）、头孢美唑（CMZ）、舒巴坦/头孢哌酮（SBT/CPZ）、交沙霉素（JM）、氯德霉素（CLDM）、米诺环素（MINO）和庆大霉素（GM）的有效率相对较低，耐药率较高。
