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本文引用的文献

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Smallpox: a potential agent of bioterrorism.天花：一种潜在的生物恐怖主义制剂。
Antiviral Res. 2003 Jan;57(1-2):7-12. doi: 10.1016/s0166-3542(02)00195-x.
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Visualizing priming of virus-specific CD8+ T cells by infected dendritic cells in vivo.在体内观察受感染的树突状细胞对病毒特异性CD8 + T细胞的启动作用。
Nat Immunol. 2002 Mar;3(3):265-71. doi: 10.1038/ni762. Epub 2002 Feb 4.
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Intracellular localization of proteasomal degradation of a viral antigen.病毒抗原蛋白酶体降解的细胞内定位
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Green fluorescent protein expressed by a recombinant vaccinia virus permits early detection of infected cells by flow cytometry.重组痘苗病毒表达的绿色荧光蛋白可通过流式细胞术早期检测受感染的细胞。
J Immunol Methods. 1998 Nov 1;220(1-2):115-21. doi: 10.1016/s0022-1759(98)00156-2.
5
Neutralizing epitope on penetration protein of vaccinia virus.牛痘病毒穿透蛋白上的中和表位
Virology. 1996 Jun 15;220(2):491-4. doi: 10.1006/viro.1996.0337.
6
E. coli beta-glucuronidase (GUS) as a marker for recombinant vaccinia viruses.
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General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes.表达外源基因的感染性痘苗病毒重组体的生产和选择通用方法。
J Virol. 1984 Mar;49(3):857-64. doi: 10.1128/JVI.49.3.857-864.1984.
8
Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques.痘苗病毒表达载体：β-半乳糖苷酶的共表达可对重组病毒噬斑进行可视化筛选。
Mol Cell Biol. 1985 Dec;5(12):3403-9. doi: 10.1128/mcb.5.12.3403-3409.1985.

基于绿色荧光蛋白流式细胞术检测的痘苗病毒中和试验的开发与应用。

Development and use of a vaccinia virus neutralization assay based on flow cytometric detection of green fluorescent protein.

作者信息

Earl Patricia L, Americo Jeffrey L, Moss Bernard

机构信息

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0445, USA.

出版信息

J Virol. 2003 Oct;77(19):10684-8. doi: 10.1128/jvi.77.19.10684-10688.2003.

DOI:10.1128/jvi.77.19.10684-10688.2003
PMID:12970455
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228521/
Abstract

A rapid and sensitive neutralization assay is required to evaluate alternative smallpox vaccines. Here we describe the development and use of a 96-well plate, semi-automated, flow cytometric assay that uses a recombinant vaccinia virus expressing enhanced green fluorescent protein and which would be applicable to other viruses.

摘要

需要一种快速且灵敏的中和试验来评估替代天花疫苗。在此，我们描述了一种96孔板、半自动流式细胞术检测方法的开发与应用，该方法使用表达增强型绿色荧光蛋白的重组痘苗病毒，并且可应用于其他病毒。