Butenko Melinka A, Patterson Sara E, Grini Paul E, Stenvik Grethe-Elisabeth, Amundsen Silja S, Mandal Abul, Aalen Reidunn B
Division of Cell and Molecular Biology, University of Oslo, N-0315 Oslo, Norway.
Plant Cell. 2003 Oct;15(10):2296-307. doi: 10.1105/tpc.014365. Epub 2003 Sep 5.
Abscission is an active process that enables plants to shed unwanted organs. Because the purpose of the flower is to facilitate pollination, it often is abscised after fertilization. We have identified an Arabidopsis ethylene-sensitive mutant, inflorescence deficient in abscission (ida), in which floral organs remain attached to the plant body after the shedding of mature seeds, even though a floral abscission zone develops. The IDA gene, positioned in the genomic DNA flanking the single T-DNA present in the ida line, was identified by complementation. The gene encodes a small protein with an N-terminal signal peptide, suggesting that the IDA protein is the ligand of an unknown receptor involved in the developmental control of floral abscission. We have identified Arabidopsis genes, and cDNAs from a variety of plant species, that encode similar proteins, which are distinct from known ligands. IDA and the IDA-like proteins may represent a new class of ligands in plants.
脱落是一个使植物能够去除不需要的器官的主动过程。由于花的目的是促进授粉，所以它通常在受精后脱落。我们鉴定出了一种拟南芥乙烯敏感突变体，即花序脱落缺陷型（ida），在该突变体中，即使形成了花脱落区，成熟种子脱落后花器官仍附着在植物体上。通过互补作用鉴定出了位于ida品系中单个T-DNA侧翼基因组DNA中的IDA基因。该基因编码一种带有N端信号肽的小蛋白，这表明IDA蛋白是参与花脱落发育控制的未知受体的配体。我们已经鉴定出了拟南芥基因以及来自多种植物物种的cDNA，它们编码相似的蛋白，这些蛋白与已知配体不同。IDA和类IDA蛋白可能代表了植物中的一类新配体。
