Van Gelder Russell N
Department of Ophthalmology and Visual Sciences, Department of Molecular Biology and Pharmacology, Washington University Medical School, Campus Box 8096, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
Retina. 2003 Aug;23(4):445-52. doi: 10.1097/00006982-200308000-00001.
The polymerase chain reaction (PCR) is a powerful molecular biologic technique that permits detection and identification of infinitesimal quantities of DNA. It is well suited to the diagnosis of infectious posterior segment ocular inflammatory disease. In this review, the basic biochemistry of PCR, indications for its use, strengths and limitations to the technique, and examples of clinical application of PCR to posterior segment disease will be discussed. Emerging uses of PCR in linking pathogen to disease will also be outlined. After completion of this article, the reader will be able to describe the basic biochemistry and practical application of PCR in the diagnosis of posterior segment inflammatory disease, list the indications, advantages, and disadvantages of the technique, and outline the application of PCR diagnostics to individual differential diagnoses.
聚合酶链反应（PCR）是一种强大的分子生物学技术，可用于检测和鉴定极微量的DNA。它非常适合用于诊断感染性眼后段炎性疾病。在这篇综述中，将讨论PCR的基本生物化学原理、其使用指征、该技术的优点和局限性，以及PCR在眼后段疾病临床应用的实例。还将概述PCR在将病原体与疾病联系起来方面的新兴用途。读完本文后，读者将能够描述PCR在眼后段炎性疾病诊断中的基本生物化学原理和实际应用，列出该技术的指征、优点和缺点，并概述PCR诊断在个体鉴别诊断中的应用。
