Cui Tianpen, Wang Lin, Wu Jianmin, Hu Lihua, Xie Jungang
Immunology Laboratory, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
J Huazhong Univ Sci Technolog Med Sci. 2003;23(2):134-7. doi: 10.1007/BF02859936.
The relationship between 3 polymorphisms sites [interleulin-4 (IL-4), IL-4 receptor (IL-4R) alpha chain and activation-induced cytidine deaminase (AICDA)] and adult allergic asthma in China was studied. By using case-control method, DNA and clinical data were obtained from allergic asthmatic patients and compared with those in the control subjects. The subjects were genotyped for the IL-4 C-589T promoter polymorphism, the IL-4R alpha chain Q576R and the AICDA C8408T by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The results showed that the IL-4 C-589T was not associated with adult allergic asthma in China. However, the IL-4R alpha chain 576R/R and AICDA 8408T/T frequency was significantly increased in allergic asthma group as compared with that in the control group [odd ratio (OR) = 3.797 and 9.127, respectively; P < 0.01)] and was correlated with the increased plasma total IgE. These data suggested that the IL-4R alpha chain 576R/R and AICDA 8408T/T genotypes confer genetic susceptibility to adult allergic asthma in China.
研究了中国人群中3个多态性位点[白细胞介素-4（IL-4）、IL-4受体（IL-4R）α链和活化诱导的胞苷脱氨酶（AICDA）]与成人过敏性哮喘之间的关系。采用病例对照法，获取过敏性哮喘患者的DNA和临床数据，并与对照组进行比较。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对研究对象进行IL-4 C-589T启动子多态性、IL-4Rα链Q576R和AICDA C8408T基因分型。结果显示，IL-4 C-589T与中国成人过敏性哮喘无关。然而，与对照组相比，过敏性哮喘组中IL-4Rα链576R/R和AICDA 8408T/T的频率显著增加[比值比（OR）分别为3.797和9.127；P<0.01]，且与血浆总IgE升高相关。这些数据表明，IL-4Rα链576R/R和AICDA 8408T/T基因型赋予中国成人过敏性哮喘遗传易感性。
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