白细胞介素-4受体基因Q576R多态性与哮喘儿童血清IgE水平的相关性

[Association of Q576R polymorphism in the interleukin-4 receptor gene with serum IgE levels in children with asthma].

作者信息

Zhang Ai-Min, Li Hai-Lin, Hao Ping, Chen Yan-Hua, Li Ji-Hai, Mo Ya-Xiong, Dai Mei

机构信息

Department of Pediatrics, People's Hospital of Hunan Province, Changsha 410005, China.

出版信息

Zhongguo Dang Dai Er Ke Za Zhi. 2006 Apr;8(2):109-12.

PMID:16613701

Abstract

OBJECTIVE

Interleukin-4 plays a key role in the development of asthma. Overseas studies have shown that Q576R polymorphism in the interleukin-4 receptor (IL-4R) gene is related to asthma as well as increased serum IgE levels. This study was designed to investigate the association of Q576R polymorphism in IL-4R gene with childhood asthma and serum IgE levels.

METHODS

The polymorphism of IL-4R Q576R was determined by PCR/RFLP and serum total IgE level was measured using ELISA in 94 children with asthma. Sixty-eight healthy children served as controls.

RESULTS

The distribution frequency of heterozygous genotype Q576R (41%) and mutant allele R576 (26%) was significantly higher in children with asthma than that of controls (16% each) (P < 0.01; P < 0.05). The total serum IgE level between patients with genotype Q576R and Q576Q was not significantly different (225.78 +/- 51.43 IU/mL vs 163.24 +/- 31.32 IU/mL, P> 0.05).

CONCLUSIONS

The mutant R576 allele of IL-4R may be one of the candidate genes for susceptibility to asthma. Allele R576 of IL-4R is related to asthma but is irrelevant to the total serum IgE level in children with asthma.

摘要

目的

白细胞介素-4在哮喘的发生发展中起关键作用。国外研究表明，白细胞介素-4受体（IL-4R）基因中的Q576R多态性与哮喘以及血清IgE水平升高有关。本研究旨在探讨IL-4R基因Q576R多态性与儿童哮喘及血清IgE水平的关系。

方法

采用PCR/RFLP法检测94例哮喘患儿IL-4R Q576R的多态性，用ELISA法检测血清总IgE水平。68例健康儿童作为对照。

结果

哮喘患儿杂合基因型Q576R的分布频率（41%）和突变等位基因R576的分布频率（26%）显著高于对照组（均为16%）（P<0.01；P<0.05）。基因型为Q576R和Q576Q的患者血清总IgE水平差异无统计学意义（225.78±51.43 IU/mL对163.24±31.32 IU/mL，P>0.05）。