Ning G, Qian J, Guo M
Department of Radiation Medicine, Second Military Medical University, Shanghai, China.
Chin J Biotechnol. 1992;8(2):131-7.
We report here that the constitute component of Na alginate is a very important factor on microcapsular strength. We developed a method of estimating viable cell count in batch microcapsular process and the factors influencing the full loading rate in microcapsules. The physical integrity of microcapsule and full loading rate were greater than 95%. The cell line of C3 hybridoma was found to reach maximum intracapsule cell density of 8.8 x 10(6) - -2.1 x 10(7) cells/ml during static culture period of 6-10 days. During this period, the mouse monoclonal antibody accumulated in the intracapsular space to a final concentration of 379 micrograms/ml. Analysis of intracapsular protein by SDS gel electrophoresis during the culture period demonstrated that there was one band or two bands of protein which means its purity is relatively high.
我们在此报告，海藻酸钠的组成成分是影响微胶囊强度的一个非常重要的因素。我们开发了一种在分批微胶囊化过程中估算活细胞数的方法以及影响微胶囊中完全装载率的因素。微胶囊的物理完整性和完全装载率均大于95%。发现在6至10天的静态培养期内，C3杂交瘤细胞系在微胶囊内达到的最大细胞密度为8.8×10⁶至2.1×10⁷个细胞/毫升。在此期间，小鼠单克隆抗体在微胶囊内空间积累至最终浓度为379微克/毫升。在培养期间通过SDS凝胶电泳对微胶囊内蛋白质进行分析表明，存在一条或两条蛋白质条带，这意味着其纯度相对较高。
