伯氏疟原虫感染的红细胞裂解物与中性红相互作用的分光光度测定法。

Spectrophotometric assay of the interaction of Plasmodium berghei infected erythrocyte lysates and neutral red.

作者信息

Dumitrescu M, Hillebrand M, Volanschi E, Panaitescu D, Stefanoiu V

机构信息

Romanian Academy, Institute of Virology St. S. Nicolau, Bucharest.

出版信息

Roum Arch Microbiol Immunol. 1992 Jul-Sep;51(3):193-203.

PMID:1297467

Abstract

In order to reveal by absorption spectrophotometry the redox differences between the Plasmodium berghei infected erythrocyte lysates (MEL) and the healthy ones (HEL) we studied their interaction with the neutral red (NR) redox dye. The variation of the dye absorption intensity at 540 nm as a function of the hemoglobin content of the samples was attributed to the redox potential variation of the different hemoglobin aggregates formed in the samples containing different hemoglobin quantities. By short term treatment of the lysates with ascorbic acid in the presence of NR, great redox reactivity difference was proved between MEL and HEL as revealed by absorption and explained by the initial higher oxidation state of the Hb iron in MEL than in HEL.

摘要

为了通过吸收分光光度法揭示伯氏疟原虫感染的红细胞裂解物（MEL）和健康红细胞裂解物（HEL）之间的氧化还原差异，我们研究了它们与中性红（NR）氧化还原染料的相互作用。染料在540nm处的吸收强度随样品血红蛋白含量的变化，归因于不同血红蛋白含量样品中形成的不同血红蛋白聚集体的氧化还原电位变化。通过在NR存在下用抗坏血酸对裂解物进行短期处理，吸收结果显示MEL和HEL之间存在很大的氧化还原反应性差异，这可以解释为MEL中Hb铁的初始氧化态高于HEL。